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The Maltese First Division knock-out 2007–08 (known as Maltco Lotteries First Division Knock-out 2007–08 due to sponsorship reasons) was a competition for Maltese football clubs from the First Division. The winners were Dingli and the runners-up were Vittoriosa.
The competition began on 18 November 2007 and ended on 1 June 2008 with the final.
The competition began with a preliminary round. Four teams played in the preliminary round. The two winners of the preliminary round advanced to the quarterfinals with the rest teams from the 1st Division. The four winners from the quarterfinals, which were Dingli, Mosta, Tarxien and Vittoriosa, advanced to the semifinals.
