Single-cell DNA template strand sequencing, or
Strand-seq, is a technique for the selective
sequencing of a daughter cell's parental template strands.
[1]
This technique offers a wide variety of applications, including the identification of sister
chromatid exchanges in the parental cell prior to segregation, the assessment of non-random segregation of sister chromatids, the identification of misoriented
contigs in
genome assemblies, de novo
genome assembly of both
haplotypes in
diploid organisms including humans, whole-chromosome
haplotyping, and the identification of germline and somatic genomic
structural variation, the latter of which can be detected robustly even in single cells.