Casamino acid

Casamino acids is a mixture of amino acids and some very small peptides obtained from acid hydrolysis of casein.^[1] It is typically used in microbial growth media. It has all the essential amino acids except tryptophan, which is destroyed by digestion with sulfuric or hydrochloric acid.^[1]

Casamino acids is similar to tryptone, the latter differing by being an incomplete enzymatic hydrolysis with some oligopeptides present, while casamino acids is predominantly free amino acids.

Uses[]

Casamino acids supplies a completely hydrolyzed protein nitrogen source. It contains a small amount of cystine. Tryptophan and vitamins are destroyed by the acid treatment. The remaining amino acids (in varying amounts) are a source of nutrients for various microorganisms. Amino acids are highly soluble and suitable for use in tissue culture. Salt content is typically 30-40%. Casamino acids are either found in the Daptacel brand DTaP vaccine or used in its manufacture.^[2]

Appearance[]

White to light tan, homogenous, free flowing powder
Solubility (2%)
Colourless to light yellow, clear in solution

References[]

^ ^a ^b Mueller, J. Howard; Johnson, Everett R (1 Jan 1941). "Acid Hydrolysates of Casein to Replace Peptone in the Preparation of Bacteriological Media". Journal of Immunology. 40 (1): 33–38. ISSN 1550-6606.
^ "Archived copy" (PDF). Archived (PDF) from the original on 2011-02-05. Retrieved 2017-09-09.{{cite web}}: CS1 maint: archived copy as title (link)

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[jimmunol-1] Mueller, J. Howard; Johnson, Everett R (1 Jan 1941). "Acid Hydrolysates of Casein to Replace Peptone in the Preparation of Bacteriological Media". Journal of Immunology. 40 (1): 33–38. ISSN 1550-6606.

[2] "Archived copy" (PDF). Archived (PDF) from the original on 2011-02-05. Retrieved 2017-09-09.{{cite web}}: CS1 maint: archived copy as title (link)

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