Excisionase
| Excisionase-like protein | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 crystal structure of the lambda xis-dna complex | |||||||||
| Identifiers | |||||||||
| Symbol | Exc | ||||||||
| Pfam | PF07825 | ||||||||
| Pfam clan | CL0123 | ||||||||
| InterPro | IPR012884 | ||||||||
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In molecular biology, excisionase is a bacteriophage protein encoded by the Xis gene. It is involved in excisive recombination by regulating the assembly of the excisive intasome and by inhibiting viral integration. It adopts an unusual winged-helix structure in which two alpha helices are packed against two extended strands. Also present in the structure is a two-stranded anti-parallel beta-sheet, whose strands are connected by a four-residue wing. During interaction with DNA, helix alpha2 is thought to insert into the major groove, while the wing contacts the adjacent minor groove or phosphodiester backbone. The C-terminal region of excisionase is involved in interaction with phage-encoded integrase (Int), and a putative C-terminal alpha helix may fold upon interaction with Int and/or DNA.[1]
References[]
- ^ Sam MD, Papagiannis CV, Connolly KM, Corselli L, Iwahara J, Lee J, Phillips M, Wojciak JM, Johnson RC, Clubb RT (December 2002). "Regulation of directionality in bacteriophage lambda site-specific recombination: structure of the Xis protein". J. Mol. Biol. 324 (4): 791–805. doi:10.1016/S0022-2836(02)01150-6. PMID 12460578.
- Protein domains