FluoProbes

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  Fluorescent dye Color mass (g/mol) Absorb (nm) Emit (nm) ε (M−1cm−1)
FluoProbes 390 violet 343 390 479 24 000
FluoProbes 488 green 804 493 519 85 000
FluoProbes 532 yellow 765 532 553 117 000
FluoProbes547H orange 736 557 574 150 000
FluoProbes 594 red 1137 601 627 120 000
FluoProbes647H far-red 761 653 674 250 000
FluoProbes 682 far-red 853 690 709 140 000
FluoProbes 752 near-IR 879 748 772 270 000
FluoProbes 782 near-IR 976 783 800 170 000
Abs = absorption maximum#, Em = emission maximum# ..................................[1]
ε = molar extinction coefficient

The FluoProbes series of fluorescent dyes were developed by Interchim to improve performances of standard fluorophores. They are designed for labeling biomolecules, cells, tissues or beads[2] in advanced fluorescent detection techniques.

  • FluoProbes dyes are typically used to label proteins or nucleic acids (ultrafast -3 minutes- labeling of antibodies employs Lightning technology[3]). Labeled products can be used for multiparameter detections, life time resolved fluorescence (TRF), polarisation anisotropy fluorescence, FRET, Quenching, FRAP. They are typically used in biotechnology and research applications as fluorescence microscopy,[4] cell biology or molecular biology, as well as infrared imaging. Derivatives with Amine and Carboxyl suit peptide and nucleic acid synthesis, while reactive ones (with succinimidyl, maleimide and hydrazide) suit conjugation by conventional chemistry, while FluoProbes labeled antibodies and cellular probes (i.e. Phalloidin) suit direct use in immunoassays or cell assays.
  • FluoProbes dyes that have comparable excitation and emission spectra to standard fluorophores such as fluoresceins, rhodamines, cyanines Cy2/3/5/5.5/7, are claimed to solve limiting issues observed in some applications such as too high background, insufficient polarity, photobleaching, insufficient brightness, or pH-sensitivity. I.e., FluoProbes488 reduces the background in Flow Cytometry[5] and in slide microscopy allowing sharper and brighter images.[6] FluoProbes 488, 547H and 647H are found more photostable [7] that is taken to good account in applications using long illuminations periods (i.e. scanning such as in confocal microscopy),[8] or for longer shelf-life of reagents (i.e. manufacturing diagnostics).
  FluoProbes dye Color Light sources (spectral line)
FluoProbes 390 violet Diode laser
FluoProbes 488
Fluorescein(FITC)/Cy2		 cyan Argon laser (488.0nm), Krypton laser (482.5nm)
FluoProbes 532 yellow Helium–neon laser (632.8nm)
FluoProbes 547H
TRITC/Cy3		 orange Argon laser (528.7nm)
FluoProbes 594
SR101/TR		 red Argon laser (528.7nm)
FluoProbes 647H
Cy5		 far red Krypton laser (647.1nm), Laser (633nm)
[9]
  • The excitation and emission spectra of the FluoProbes series covers much of the visible spectrum up to the infrared region, matching the commonly used light sources and filters.

Similar lines of fluorescent dyes provide an alternative to the FluoProbes Dyes.

References[]

  1. ^ "FluoProbes Dyes" (PDF). Interchim. 2010. Retrieved 2010-03-04.
  2. ^ Article Savina A. ; Cell 126, 205–218, July 14, 2006 (Phagosome Neutrality in Host Defense)
  3. ^ Lightning technology from Innova BioSciences
  4. ^ Article Brunner ; Molecular & Cellular Proteomics 2007, 6.6, pp.1007-1017
  5. ^ AnnexinV-FluoProbes488 comparison in FCM
  6. ^ FluoProbes labeling agent
  7. ^ FluoProbes488 comparison to FITC, Cyanine2
  8. ^ FluoProbes547H comparison in Confocal Microscopy
  9. ^ "FluoProbes Dyes" (PDF). Interchim. 2010. Retrieved 2010-03-01.
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