Grancalcin is a protein that in humans is encoded by the GCA gene .[5] [6] [7] [8]
This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.[8]
Interactions [ ]
GCA (gene) has been shown to interact with SRI .[7]
References [ ]
^ Jump up to: a b c GRCh38: Ensembl release 89: ENSG00000115271 - Ensembl , May 2017
^ Jump up to: a b c GRCm38: Ensembl release 89: ENSMUSG00000026893 - Ensembl , May 2017
^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ Boyhan A, Casimir CM, French JK, Teahan CG, Segal AW (Mar 1992). "Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes" . J Biol Chem . 267 (5): 2928–33. doi :10.1016/S0021-9258(19)50675-5 . PMID 1737748 .
^ Teahan CG, Totty NF, Segal AW (Oct 1992). "Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils" . Biochem J . 286. ( Pt 2) (2): 549–54. doi :10.1042/bj2860549 . PMC 1132932 . PMID 1530588 .
^ Jump up to: a b Hansen C, Tarabykina S, la Cour JM, Lollike K, Berchtold MW (Jun 2003). "The PEF family proteins sorcin and grancalcin interact in vivo and in vitro" . FEBS Lett . 545 (2–3): 151–4. doi :10.1016/S0014-5793(03)00518-0 . PMID 12804766 . S2CID 42572647 .
^ Jump up to: a b "Entrez Gene: GCA grancalcin, EF-hand calcium binding protein" .
Further reading [ ]
Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration" . Cell . 125 (4): 801–14. doi :10.1016/j.cell.2006.03.032 . PMID 16713569 . S2CID 13709685 .
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature . 437 (7062): 1173–8. Bibcode :2005Natur.437.1173R . doi :10.1038/nature04209 . PMID 16189514 . S2CID 4427026 .
Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4" . Nature . 434 (7034): 724–31. Bibcode :2005Natur.434..724H . doi :10.1038/nature03466 . PMID 15815621 .
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 . PMID 15489334 .
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. doi :10.1073/pnas.242603899 . PMC 139241 . PMID 12477932 .
Jia J, Borregaard N, Lollike K, Cygler M (2002). "Structure of Ca(2+)-loaded human grancalcin" . Acta Crystallogr. D . 57 (Pt 12): 1843–9. doi :10.1107/S0907444901016511 . PMID 11717497 .
Lollike K, Johnsen AH, Durussel I, et al. (2001). "Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner" . J. Biol. Chem . 276 (21): 17762–9. doi :10.1074/jbc.M100965200 . PMID 11279160 .
Jia J, Han Q, Borregaard N, et al. (2000). "Crystal structure of human grancalcin, a member of the penta-EF-hand protein family". J. Mol. Biol . 300 (5): 1271–81. doi :10.1006/jmbi.2000.3925 . PMID 10903868 .
Maki M, Narayana SV, Hitomi K (1998). "A growing family of the Ca2+-binding proteins with five EF-hand motifs" . Biochem. J . 328. ( Pt 2): 718–20. PMC 1218977 . PMID 9441591 .
show PDB gallery
1f4o : CRYSTAL STRUCTURE OF GRANCALCIN WITH BOUND CALCIUM
1f4q : CRYSTAL STRUCTURE OF APO GRANCALCIN
1k94 : Crystal structure of des(1-52)grancalcin with bound calcium
1k95 : Crystal structure of des(1-52)grancalcin with bound calcium