June Almeida

From Wikipedia, the free encyclopedia

June Dalziel Almeida
June Dalziel Almeida died 2007.png
Born
June Dalziel Hart

(1930-10-05)5 October 1930
Glasgow, Scotland
Died1 December 2007(2007-12-01) (aged 77)
Bexhill, England
CitizenshipBritish
Known forPioneer of immuno-electron microscopy, identification of coronavirus, hepatitis B, HIV, rubella
Spouse(s)
Enriques Rosalio (Henry) Almeida
​
​
(m. 1954, divorced)​

Phillip Samuel Gardner
​
​
(m. 1982; died 1994)​
Children1
Scientific career
FieldsVirology, histopathology
InstitutionsGlasgow Royal Infirmary, St Bartholomew's Hospital, Ontario Cancer Institute, Royal Postgraduate Medical School, Wellcome Research Laboratories
InfluencedAlbert Kapikian and Thomas Henry Flewett and Hugh Pennington

June Dalziel Almeida (5 October 1930 – 1 December 2007) was a Scottish virologist, a pioneer in virus imaging, identification, and diagnosis. Her skills in electron microscopy earned her an international reputation.[1][2]

In 1964, she was recruited by St Thomas's Hospital Medical School in London. By 1967, she had earned her Doctor of Science (Sc.D.) on the basis of her research and the resulting publications, while working in Canada, at Toronto's Ontario Cancer Institute and then in London at St Thomas's.[1] she then continued her research at the Royal Postgraduate Medical School (RPGMS),[1] which later became part of the Imperial College School of Medicine.

Almeida succeeded in identifying viruses that were previously unknown, including—in 1966—a group of viruses that was later named coronavirus,[Publications 1][3]:96 due to their crown-like appearance.[4] her immune electron microscopy (IEM) innovations and insights contributed to research related to the diagnosis of hepatitis B, HIV, and rubella, among other viral diseases. Her electron micrographs continue to be included in virology review textbooks, decades after she produced them.[1]

Early life[]

June Dalziel Hart was born on 5 October 1930 at 10 Duntroon Street, Glasgow to Jane Dalziel (née Steven) and Harry Leonard Hart, a bus driver.[1]:1511.1–1511[5][2][6][full citation needed] In 1940, her 6-year-old brother died of diphtheria, perhaps leading to her interest in diseases.[4]

In 1947, when she was 16, Hart left Whitehill Secondary school, and despite her academic strength, winning the science prize,[4] her family did not have the financial means to allow her to attend university at that time.[1] She began working as a histopathology technician, first, at the Glasgow Royal Infirmary, and was then recruited by a former colleague, Dr. John WS Blacklock [4] to do similar work at St Bartholomew's Hospital, where she worked until 1954.[1] She married Enriques Rosalio (Henry) Almeida in 1954; they later divorced.

Career[]

Ontario Cancer Institute[]

In 1954, Almeida was hired for a newly opened position as electron microscopy technician at the Ontario Cancer Institute,[1][5]:209 where she worked for ten years.

While working as an electron microscopist, she and her Cancer Institute colleagues produced a series of studies applying negative staining to clinical problems.[5]:209

In 1963, she was the first of three authors of an article in the journal Science, in which they identified virus-like particles in cancer patients' blood.[7] In the same year, she published her research in which she "negatively stained aggregates of antigen...and antibody" with the electron microscope.[Publications 2] Almeida's sense of humour arose in an ode to electron microscopy, and the symmetrical structure of coronavirus (with an apology to poet, William Blake).

“Virus, virus shining bright,

In the phosphotungstic night,

What immortal hand or eye,

Dare frame thy fivefold symmetry.”[4][2]

St Thomas's Hospital Medical School[]

Main article: St Thomas's Hospital Medical School

By 1964, Almeida had earned her Doctor of Science (Sc.D.), based on publications of her research at the Ontario Cancer Institute and at St Thomas's on electron micrographic research of antibodies.[5]:209

In 1964, Tony Waterson, who had just been appointed as chair of microbiology at St Thomas's, met Almeida while visiting Toronto, and recruited her to join his research team[3]:96 at one of the oldest and most prestigious medical schools in the United Kingdom—London's St Thomas's Hospital Medical School, now part of King's College London. At St Thomas's, she worked on hepatitis B virus and the cold viruses.[8]

In 1966, Waterson and Almeida collaborated with the physician and director of research on the common cold, David Tyrrell, who was working on a new organ culture system. Tyrrell's team had been attempting to detect the presence of rhinoviruses in tissue cultures of cells that they had produced in the lab. They wanted to detect a specific respiratory virus they called B814. The Swedish professor Bertil Hoorn could make all Tyrrell's respiratory viruses in organ cultures of cells from the human airway in the laboratory, except for virus B814. This organ culture system meant they did not have to depend on human volunteers to do research on these viruses. They wanted a reliable method to detect virus B814.[3]:94

In the book Cold Wars (2002), which Tyrrell wrote with Michael Fielder, he described how when he first met Almeida she seemed to be extending the range of the electron microscope to new limits.[3]:96

According to Tyrrell, prior to her innovative work, it was generally accepted that viruses had to be concentrated and purified to detect them with the electron microscope. When she told Tyrrell that she could "find virus particles" in the organ cultures that they had collected, with her "new, improved techniques", he was skeptical.[3]:96

Tyrrell's team sent samples to Almeida in London. These included a sample prepared with the B814 virus, along with samples infected with influenza and herpes, which were well-known. When she examined the samples through her microscope grids, "she recognized all the known viruses, and her pictures revealed their structure beautifully. But more importantly, she also saw virus particles in the B814 sample."[3]:96 she told Tyrrell that the B814 specimens had reminded her of particles she had previously studied in a "disease called infectious bronchitis of chickens" and in another disease—"mouse hepatitis liver inflammation." Almeida's papers on these had been rejected because the referees considered her electron micrographs to be "bad pictures" of known influenza virus particles. Almeida told him she now knew that these "three viruses were something quite new."[3]:96

According to Tyrrell, once Almeida had identified the previously unrecognised group of viruses, they met in Waterson's office, to decide on its name. The viruses appeared to be surrounded by a "halo", which in Latin is "corona", and the name "coronavirus" was born.[3]:96

In 1966, she and Tyrrell wrote that "The particles are pleomorphic, in the size range 800 to 1200 Å, and are surrounded by a distinct 200 Å long fringe. They are indistinguishable from the particles of avian infectious bronchitis, the only virus previously known to have this morphology."[Publications 1]

Royal Postgraduate Medical School of London (RPGMS)[]

Three years later, in 1967, when Waterson took a position at the Royal Postgraduate Medical School (RPGMS), Almeida also moved to begin work there.

In 1968, she published an article in Journal of General Virology, on "avian infectious bronchitis virus".[Publications 3]

In 1971, using her immune electronmicroscopy technique, Almeida made the landmark discovery that the hepatitis B virus had "two immunologically distinct components"—an "outer coat and a small inner component".[9]

Wellcome Institute[]

Almeida spent her final years of her professional career at the Wellcome Institute before she retired.[8] While working for Wellcome, she was named on several patents in the field of imaging viruses.[10]

Major contributions[]

In their 2013 book entitled To Catch a Virus, John Booss and Marilyn J. August describe how Almeida "played a crucial role in adapting the electron microscope to clinical diagnostic virology work."[5]:209[Notes 1]

Before her work with Anthony Peter Waterson in the 1960s, very few improvements had been made on the initial 1941 "proof of principle aggregation of virus by virus-specific antibody observable by [electron microscope (EI)]." In 1963, she pioneered a technique in immune electron microscopy (IEM), to better visualise viruses by using antibodies to aggregate them.[Publications 2] In the 1960s, she and Waterson were using negative staining for the EM of viruses—a technique that was both rapid and simple—and provided excellent detailed observations of viral morphology, which had revolutionized the electron microscopy of viruses overnight.[5]:208

In 1966, using her new techniques, Almeida was able to identify a group of "previously uncharacterised human respiratory viruses", while collaborating with David Tyrrell,[3]:96[5]:209 then director of the Common Cold Research Centre[1] in Salisbury in Wiltshire.[11] Tyrrell suggested calling the new group "coronaviruses". The coronavirus family of viruses now includes the SARS virus[1] and the SARS-CoV2 virus that causes Coronavirus disease 2019.[3]:96

In 1967, using the IEM aggregation method, Almeida produced the first visualization of rubella virus.[1][12][13]

In retirement[]

After taking early retirement[4] from Wellcome Institute, Almeida returned in an advisory role at St. Thomas's in the late 1980s when she helped produce micrographs of the HIV virus,[1] where Aberdeen professor of bacteriology Hugh Pennington was also then attached.[4]

Almeida's publications include the 1979 Manual for rapid laboratory viral diagnosis for the World Health Organization.[Publications 4]

Almeida also trained as a yoga teacher and became involved in her second husband Philip Gardner's antique business (he died in 1994).[2]

Legacy[]

At the Royal Postgraduate Medical School (RPGMS), in 1970, Almeida taught Albert Kapikian the technique of immune electron microscopy (IEM). Kapikian, who was visiting for six months from the United States National Institutes of Health, used her techniques in the identification of a cause of non-bacterial gastroenteritis—the Norwalk virus, now known as the Norovirus.[8][1]

Almeida's work received new attention during the early months of the COVID-19 pandemic. Her little-known story was first published by the Herald in Scotland on 7 March, the BBC on 15 April,[11] the National Geographic on April 17,[14] and later her research noted in The National on 19 May 2020.[4] According to professor of bacteriology Hugh Pennington, Chinese scientists credited Almeida's work, including techniques she developed, with the early identification of Coronavirus disease 2019.[15]

Personal life[]

Almeida moved to Toronto, Ontario and worked at the Ontario Cancer Institute of the Princess Margaret Cancer Centre after her marriage on 11 December 1954 to Enrique Rosalio (Henry) Almeida (1913–1993), a Venezuelan artist with whom she had a daughter, Joyce.

Her daughter Joyce, a psychiatrist, has two daughters.[1] Almeida's first marriage ended in divorce.

She retired in 1985 to Bexhill-on-Sea with her second husband, Phillip Samuel Gardner, a fellow virologist,[1] whom she had married in 1979,[16] and who died in 1994.[2]

She died in Bexhill from a heart attack in 2007.[8]

Commemoration[]

A new COVID-19 testing laboratory in Guy's Hospital was named after June Almeida in September 2020.[17]

See also[]

Notes[]

  1. ^ In the chapter entitled "Imaging viruses and tagging their antigens" in John Booss and Marilyn J. August's 2013 To Catch a Virus, they describe the how "refinements in electron microscopy (EM)...greatly expanded the understanding of the structure and replication of viruses and facilitated the application of EM to viral diagnosis. The refinements included thin sectioning, negative staining, and immunoelectron microscopy (IEM) developed for clinical diagnostic work. The use of EM in discovering the viral causes of acute gastroenteritis and infectious hepatitis are then considered." ASM: "This chapter first examines the refinements in electron microscopy (EM) that greatly expanded the understanding of the structure and replication of viruses and facilitated the application of EM to viral diagnosis. The refinements included thin sectioning, negative staining, and immunoelectron microscopy (IEM) developed for clinical diagnostic work. The use of EM in discovering the viral causes of acute gastroenteritis and infectious hepatitis are then considered."

Selected publications[]

  1. ^ Jump up to: a b Almeida, June D.; Tyrrell, D. A. J. (1967). "The Morphology of Three Previously Uncharacterized Human Respiratory Viruses that Grow in Organ Culture". Journal of General Virology. 1 (2): 175–178. doi:10.1099/0022-1317-1-2-175. ISSN 0022-1317. PMID 4293939.
  2. ^ Jump up to: a b Almeida, June; Cinader, Bernhard; Howatson, Allan (1 September 1963). "The Structure of Antigen-Antibody Complexes: A Study by Electron Microscopy". Journal of Experimental Medicine. 118 (3): 327–340. doi:10.1084/jem.118.3.327. ISSN 0022-1007. PMID 14077994. Retrieved 18 April 2020.
  3. ^ Berry, D. M.; Almeida, June D. (1968). "The Morphological and Biological Effects of Various Antisera on Avian Infectious Bronchitis Virus". Journal of General Virology (JGV). 3 (1): 97–102. doi:10.1099/0022-1317-3-1-97. ISSN 0022-1317. PMID 5692879.
  4. ^ Almeida, June D. (1979). "Manual for rapid laboratory viral diagnosis" (PDF). World Health Organization (WHO).

References[]

  1. ^ Jump up to: a b c d e f g h i j k l m n o Almeida, Joyce (28 June 2008). "June Almeida (née Hart)". The BMJ. BMJ Group. 336 (7659): 1511.1–1511. Bibcode:1963Sci...142.1487A. doi:10.1136/bmj.a434. PMC 2440895.
  2. ^ Jump up to: a b c d e Gellene, Denise (8 May 2020). "Overlooked No More: June Almeida, Scientist Who Identified the First Coronavirus". The New York Times. In 1966, she used a powerful electron microscope to capture an image of a mysterious pathogen — the first coronavirus known to cause human disease.
  3. ^ Jump up to: a b c d e f g h i j Tyrrell, David; Fielder, Michael (2002). Cold wars: the fight against the common cold. Oxford, England: Oxford University Press. p. 253.
  4. ^ Jump up to: a b c d e f g h "Scottish doctor found first human coronavirus case in 1960s". The National. Retrieved 20 May 2020.
  5. ^ Jump up to: a b c d e f g ; August, Marilyn J. (2013). "Chapter 7: Imaging viruses and tagging their antigenes". To Catch a Virus. Washington, DC: ASM Press. pp. 197–249. ISBN 978-1-55581-507-3. Retrieved 18 April 2020. ASM: "This chapter first examines the refinements in electron microscopy (EM) that greatly expanded the understanding of the structure and replication of viruses and facilitated the application of EM to viral diagnosis. The refinements included thin sectioning, negative staining, and immunoelectron microscopy (IEM) developed for clinical diagnostic work. The use of EM in discovering the viral causes of acute gastroenteritis and infectious hepatitis are then considered."
  6. ^ "The Times & The Sunday Times". www.thetimes.co.uk.
  7. ^ Almeida, June D.; Hasselback, Richard C.; Ham, Arthur W. (13 December 1963). "Virus-Like Particles in Blood of Two Acute Leukemia Patients". Science. 142 (3598): 1487–1489. Bibcode:1963Sci...142.1487A. doi:10.1126/science.142.3598.1487. PMID 14077540. Retrieved 18 April 2020.
  8. ^ Jump up to: a b c d Banatvala, J. E. (January 2011). "Oxford Dictionary of National Biography". Oxford Dictionary of National Biography. Almeida, June Dalziel (1930–2007) (online ed.). Oxford University Press. doi:10.1093/ref:odnb/99332. Retrieved 18 October 2012. (Subscription or UK public library membership required.)
  9. ^ Nahmias, André J.; O'Reilly, Richard J. (6 December 2012). Immunology of Human Infection: Part II: Viruses and Parasites; Immunodiagnosis and Prevention of Infectious Diseases. Springer Science & Business Media. ISBN 978-1-4684-1012-9.
  10. ^ Almeida, June Dalziel, patents, accessed October 2012
  11. ^ Jump up to: a b Brocklehurst, Steven (15 April 2020). "The woman who discovered the first coronavirus". BBC Scotland News. Retrieved 17 April 2020.
  12. ^ Best, Jennifer M; Banatvala, J. E.; Almeida, June D; Waterson, A. P. (29 July 1967). "Morphological characteristics of rubella virus". The Lancet. 290 (7509): 237–239. doi:10.1016/S0140-6736(67)92302-1. ISSN 0140-6736. Retrieved 18 April 2020.
  13. ^ Paterson, Andrew (2017). Brilliant! Scottish inventors, innovators, scientists and engineers who changed the world. London: Austin Macauley. p. 577. ISBN 9781786294357.
  14. ^ Combs, Sydney (17 April 2020). "She Discovered Coronaviruses Decades Ago—But Got Little Recognition". National Geographic Society. National Geographic. Retrieved 18 April 2020.
  15. ^ "June Almeida, tribute to Scotland's forgotten hero of the coronavirus". Herald Scotland. 7 March 2020. Retrieved 18 April 2020.
  16. ^ "Obituary PS Gardner". British Medical Journal. 309: 950–1. 8 October 1994.
  17. ^ Trust, Guy's and St Thomas's NHS Foundation. "Guy's Hospital names Covid lab after pioneer who discovered the virus". www.guysandstthomas.nhs.uk. Retrieved 14 September 2020.
Retrieved from ""