Karnovsky fixative
Karnovsky fixative, developed by M. J. Karnovsky, is a fixative for electron microscopy.[1]
Solution[]
The stock solution for Karnovsky fixative is as follows:[1]
- paraformaldehyde 2.0 g
- 1M sodium hydroxide 2 - 4 drops
- 50% glutaraldehyde 5.0 ml (25% glut - 10 ml)
- 0.2M cacodylate buffer, pH 7.4, 20.0 mL
Modified solutions[]
Karnovsky's fixative solution is often modified for specialized applications.[2][3] For example, 2% paraformaldehyde, 2.5% glutaraldehyde in 0.1 Molar sodium phosphate buffer (pH 7.4) has been used to study the ultrastructure of renal pelvis fragments.[3]
References[]
- ^ a b Karnovsky fixative
- ^ Shah FA, Johansson BR, Thomsen P, Palmquist A (2015). "Ultrastructural evaluation of shrinkage artefacts induced by fixatives and embedding resins on osteocyte processes and pericellular space dimensions". Journal of Biomedical Materials Research. 103 (4): 1565–1576. doi:10.1002/jbm.a.35287. PMID 25046613.
- ^ a b Favorito LA, Costa WS, Lobo M, Gallo CM, Sampaio FJ (2020). "Morphology of the fetal renal pelvis during the second trimester: Comparing genders". Journal of Pediatric Surgery. doi:10.1016/j.jpedsurg.2019.12.029. PMID 32037216.
External links[]
Categories:
- Electron microscopy