OD600

OD600 (Also written as O.D. 600, D₆₀₀, o.d. 600, OD₆₀₀) is an abbreviation indicating the optical density of a sample measured at a wavelength of 600 nm. It is a commonly used in Spectrophotometry for estimating the concentration of bacteria or other cells in a liquid as the 600nm wavelength does little to damage or hinder their growth. Optical density is largely based on light scattering and should not be confused with absorption.

Measuring the concentration can indicate the growth stage of cultured cell population, i.e. whether it is in the lag phase, log phase, or stationary phase.^[1] This is done by measuring the absorbance of the OD₆₀₀ light with the use of a Spectrophotometer. A growth curve can then be constructed by taking absorbance measurements as a function of time.

OD₆₀₀ is preferable to UV spectroscopy when measuring the growth over time of a cell population because at this wavelength, the cells will not be killed as they would under too much UV light. UV light has also been shown to cause small to medium-sized mutations in bacteria, potentially altering or destroying genes of interest.^[2]

OD₆₀₀ is a type of turbidity measurement.

References[]

^ Mandelstam J, McQuillen K, Dawes I (eds): Biochemistry of Bacterial Growth. 3rd Ed. Blackwell, Oxford, 1982 .
^ Sutton, Scott. "Measurement of Cell Concentration in Suspension by Optical Density". microbiologynetwork.com. The Microbiology Network. Retrieved 26 September 2019.

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[1] Mandelstam J, McQuillen K, Dawes I (eds): Biochemistry of Bacterial Growth. 3rd Ed. Blackwell, Oxford, 1982 .

[Network-2] Sutton, Scott. "Measurement of Cell Concentration in Suspension by Optical Density". microbiologynetwork.com. The Microbiology Network. Retrieved 26 September 2019.

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