GPI transamidase component PIG-T is an enzyme that in humans is encoded by the PIGT gene .[5] [6] [7]
This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase . GPI transamidase mediates GPI anchoring in the endoplasmic reticulum , by catalyzing the transfer of fully assembled GPI units to proteins.[6]
Interactions [ ] PIGT has been shown to interact with PIGK [8] GPAA1 .[7] [9]
References [ ]
^ a b c GRCh38: Ensembl release 89: ENSG00000124155 - Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000017721 - Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Vainauskas S, Menon AK (Apr 2005). "Endoplasmic reticulum localization of Gaa1 and PIG-T, subunits of the glycosylphosphatidylinositol transamidase complex" . J Biol Chem . 280 (16): 16402–9. doi :10.1074/jbc.M414253200 PMID 15713669 . ^ a b "Entrez Gene: PIGT phosphatidylinositol glycan anchor biosynthesis, class T" .^ a b Ohishi K, Inoue N, Kinoshita T (August 2001). "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8" . EMBO J . 20 (15): 4088–98. doi :10.1093/emboj/20.15.4088 . PMC 149153 PMID 11483512 . ^ Ohishi, Kazuhito; Nagamune Kisaburo; Maeda Yusuke; Kinoshita Taroh (Apr 2003). "Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge" . J. Biol. Chem . 278 (16): 13959–67. doi :10.1074/jbc.M300586200 ISSN 0021-9258 . PMID 12582175 . ^ Vainauskas, Saulius; Maeda Yusuke; Kurniawan Henry; Kinoshita Taroh; Menon Anant K (Aug 2002). "Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex" . J. Biol. Chem . 277 (34): 30535–42. doi :10.1074/jbc.M205402200 ISSN 0021-9258 . PMID 12052837 .
Further reading [ ]
Eisenhaber B, Maurer-Stroh S, Novatchkova M, et al. (2003). "Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins". BioEssays . 25 (4): 367–85. doi :10.1002/bies.10254 . PMID 12655644 . Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene . 138 (1–2): 171–4. doi :10.1016/0378-1119(94)90802-8 . PMID 8125298 . Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene . 200 (1–2): 149–56. doi :10.1016/S0378-1119(97)00411-3 . PMID 9373149 . Lai CH, Chou CY, Ch'ang LY, et al. (2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics" . Genome Res . 10 (5): 703–13. doi :10.1101/gr.10.5.703 . PMC 310876 PMID 10810093 . Ohishi K, Inoue N, Kinoshita T (2001). "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8" . EMBO J . 20 (15): 4088–98. doi :10.1093/emboj/20.15.4088 . PMC 149153 PMID 11483512 . Yu Y, Zhang C, Zhou G, et al. (2001). "Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs" . Genome Res . 11 (8): 1392–403. doi :10.1101/gr.175501 . PMC 311073 PMID 11483580 . Fossey SC, Mychaleckyj JC, Pendleton JK, et al. (2001). "A high-resolution 6.0-megabase transcript map of the type 2 diabetes susceptibility region on human chromosome 20". Genomics . 76 (1–3): 45–57. doi :10.1006/geno.2001.6584 . PMID 11549316 . Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20" . Nature . 414 (6866): 865–71. Bibcode :2001Natur.414..865D . doi :10.1038/414865a PMID 11780052 . Vainauskas S, Maeda Y, Kurniawan H, et al. (2002). "Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex" . J. Biol. Chem . 277 (34): 30535–42. doi :10.1074/jbc.M205402200 PMID 12052837 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Ohishi K, Nagamune K, Maeda Y, Kinoshita T (2003). "Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge" . J. Biol. Chem . 278 (16): 13959–67. doi :10.1074/jbc.M300586200 PMID 12582175 . Hong Y, Ohishi K, Kang JY, et al. (2004). "Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins" . Mol. Biol. Cell . 14 (5): 1780–9. doi :10.1091/mbc.E02-12-0794 . PMC 165076 PMID 12802054 . Clark HF, Gurney AL, Abaya E, et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment" . Genome Res . 13 (10): 2265–70. doi :10.1101/gr.1293003 . PMC 403697 PMID 12975309 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries" . DNA Res . 12 (2): 117–26. doi :10.1093/dnares/12.2.117 PMID 16303743 . Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes" . Genome Res . 16 (1): 55–65. doi :10.1101/gr.4039406 . PMC 1356129 PMID 16344560 . Li HL, Li Z, Qin LY, et al. (2006). "The novel neurotrophin-regulated neuronal development-associated protein, NDAP, mediates apoptosis" . FEBS Lett . 580 (7): 1723–8. doi :10.1016/j.febslet.2006.02.022 PMID 16516892 . S2CID 1016691 .
