POLR3F

POLR3F
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	2DK5, 2YU3
Identifiers
Aliases	POLR3F, RPC39, RPC6, polymerase (RNA) III subunit F, RNA polymerase III subunit F, C34
External IDs	OMIM: 617455 MGI: 1924086 HomoloGene: 4722 GeneCards: POLR3F
Gene location (Human)
Chr.	Chromosome 20 (human)
End	18,484,646 bp
Gene location (Mouse)
Chr.	Chromosome 2 (mouse)
End	144,383,915 bp
RNA expression pattern
	Top expressed in
	cerebellar hemisphere; ; Achilles tendon; ; caudate nucleus; ; Brodmann area 9; ; ovary; ; prosencephalon;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	DNA-directed 5'-3' RNA polymerase activity; GO:0001948 protein binding; RNA polymerase III activity; double-stranded DNA binding; DNA binding;
Cellular component	RNA polymerase III complex; cytosol; nucleus; nucleoplasm;
Biological process	positive regulation of interferon-beta production; immune system process; defense response to virus; positive regulation of innate immune response; transcription by RNA polymerase III; innate immune response; regulation of transcription by RNA polymerase III; positive regulation of type I interferon production;
	Sources:Amigo / QuickGO
Orthologs
	10621
	70408
	ENSG00000132664
	ENSMUSG00000027427
	Q9H1D9
	Q921X6
	NM_001282526; NM_006466
	NM_029763; NM_027417
	NP_001269455; NP_006457
	NP_084039
	Wikidata
View/Edit Human	View/Edit Mouse

DNA-directed RNA polymerase III subunit RPC6 is an enzyme that in humans is encoded by the POLR3F gene.^[5]^[6]

Function[]

The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.^[6]

Model organisms[]

Model organisms have been used in the study of POLR3F function. A conditional knockout mouse line called Polr3f^{tm1a(EUCOMM)Wtsi} was generated at the Wellcome Trust Sanger Institute.^[7] Male and female animals underwent a standardized phenotypic screen^[8] to determine the effects of deletion.^[9]^[10]^[11]^[12] Additional screens performed: - In-depth immunological phenotyping^[13]

*Polr3f* knockout mouse phenotype
Characteristic	Phenotype
All data available at.^[8]^[13]
Insulin	Normal
Homozygous viability at P14	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Micronucleus test	Abnormal
Salmonella infection	Normal
Epidermal Immune Composition	Normal
Influenza Challenge	Normal

References[]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000132664 - Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000027427 - Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Wang Z, Roeder RG (May 1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation". Genes & Development. 11 (10): 1315–26. doi:10.1101/gad.11.10.1315. PMID 9171375.
^ ^a ^b "Entrez Gene: POLR3F polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa".
^ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
^ ^a ^b "International Mouse Phenotyping Consortium".
^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
^ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
^ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
^ ^a ^b "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading[]

Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (Sep 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
Hu P, Wu S, Sun Y, Yuan CC, Kobayashi R, Myers MP, Hernandez N (Nov 2002). "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits" (PDF). Molecular and Cellular Biology. 22 (22): 8044–55. doi:10.1128/MCB.22.22.8044-8055.2002. PMC 134740. PMID 12391170.
Jones E, Kimura H, Vigneron M, Wang Z, Roeder RG, Cook PR (Jan 2000). "Isolation and characterization of monoclonal antibodies directed against subunits of human RNA polymerases I, II, and III". Experimental Cell Research. 254 (1): 163–72. doi:10.1006/excr.1999.4739. PMID 10623476.
Hsieh YJ, Kundu TK, Wang Z, Kovelman R, Roeder RG (Nov 1999). "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity". Molecular and Cellular Biology. 19 (11): 7697–704. doi:10.1128/mcb.19.11.7697. PMC 84812. PMID 10523658.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Jang KL, Collins MK, Latchman DS (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". Journal of Acquired Immune Deficiency Syndromes. 5 (11): 1142–7. PMID 1403646.

This article on a gene on human chromosome 20 is a stub. You can help Wikipedia by .

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000132664 - Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000027427 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9171375-5] Wang Z, Roeder RG (May 1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation". Genes & Development. 11 (10): 1315–26. doi:10.1101/gad.11.10.1315. PMID 9171375.

[entrez-6] "Entrez Gene: POLR3F polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa".

[mgp_reference-7] Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.

[IMPCsearch_ref-8] "International Mouse Phenotyping Consortium".

[pmid21677750-9] Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.

[mouse_library-10] Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.

[mouse_for_all_reasons-11] Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.

[pmid23870131-12] White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.

[iii_ref-13] "Infection and Immunity Immunophenotyping (3i) Consortium".

[1]

[2]

[3]

[4]

[5]

[6]

[7]

[8]

[9]

[10]

[11]

[12]

[13]