para-Nitrophenylphosphate
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Names | |||
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Preferred IUPAC name
4-Nitrophenyl dihydrogen phosphate | |||
Other names
pNPP
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Identifiers | |||
3D model (JSmol)
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ChEBI | |||
ChEMBL | |||
ChemSpider | |||
ECHA InfoCard | 100.005.777 | ||
PubChem CID
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CompTox Dashboard (EPA)
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Properties | |||
C6H6NO6P | |||
Molar mass | 219.09 | ||
Related compounds | |||
Related compounds
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Paraoxon | ||
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |||
what is ?) | (|||
Infobox references | |||
para-Nitrophenylphosphate (pNPP) is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.[1] Phosphatases catalyze the hydrolysis of pNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm.[2] This property can be used to determine the activity of various phosphatases including alkaline phosphatase (AP) and protein tyrosine phosphatase (PTP).[3]
The substance is sensitive to light, and thus should be stored protected from light. This is also important after adding the substrate to the mixture and before reading. −20 °C is the optimal storage temperature.[4]
References[]
- ^ Lorenz, Ulrike (2017-05-07). "Protein Tyrosine Phosphatase Assays". Current Protocols in Immunology. 91 (1): 11.7.1–12. doi:10.1002/0471142735.im1107s93. ISBN 978-0471142737. PMC 3097125. PMID 21462163.
- ^ MacKintosh, C. (1993). In D.G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach. 221. New York: IRL Press.
- ^ Matsushima, Ayako; Inoue, Yorinao; Shibata, Kazuo (1975). "Derivative absorption spectrophotometry of native proteins". Analytical Biochemistry. 65 (1–2): 362–368. doi:10.1016/0003-2697(75)90520-5. ISSN 0003-2697. PMID 1169006.
- ^ Biolabs, New England. "p-Nitrophenyl Phosphate (PNPP) - NEB". www.NEB.com. Retrieved 29 October 2017.
External links[]
Categories:
- Organophosphates
- Nitrobenzenes
- Phenol esters
- Organic compound stubs