SAE2 (yeast)

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Not to be confused with SUMO-activating enzyme subunit 2 (SAE2), the protein product of the human gene UBA2.

SAE2 is a gene in budding yeast, coding for the protein Sae2, which is involved in DNA repair. Sae2 is a part of the homologous recombination process in response to double-strand breaks.[1] It is best characterized in the yeast model organism Saccharomyces cerevisiae.[2] Homologous genes in other organisms include in fission yeast, Com1 in plants, and in higher eukaryotes including humans.[2]

Sae2 and its homologs have relatively long low-complexity regions in their primary sequences and appear to have large intrinsically unstructured regions. Sae2 likely forms tetramers through coiled-coil sequences.[2] Proteins of this family are DNA-binding proteins and are involved in DNA end resection and bridging at double-strand breaks.[3][4] Sae2 has been reported to have endonuclease activity,[5] though it has no bioinformatically recognizable nuclease sequence and reports of this activity are not consistent in the literature.[6][2]

References[]

  1. ^ Rattray, AJ; McGill, CB; Shafer, BK; Strathern, JN (May 2001). "Fidelity of mitotic double-strand-break repair in Saccharomyces cerevisiae: a role for SAE2/COM1". Genetics. 158 (1): 109–22. PMID 11333222.
  2. ^ a b c d Andres, Sara N.; Williams, R. Scott (August 2017). "CtIP/Ctp1/Sae2, molecular form fit for function". DNA Repair. 56: 109–117. doi:10.1016/j.dnarep.2017.06.013. PMC 5543718.
  3. ^ Huertas, Pablo; Cortés-Ledesma, Felipe; Sartori, Alessandro A.; Aguilera, Andrés; Jackson, Stephen P. (October 2008). "CDK targets Sae2 to control DNA-end resection and homologous recombination". Nature. 455 (7213): 689–692. doi:10.1038/nature07215. PMC 2635538.
  4. ^ Clerici, Michela; Mantiero, Davide; Lucchini, Giovanna; Longhese, Maria Pia (18 November 2005). "The Saccharomyces cerevisiae Sae2 Protein Promotes Resection and Bridging of Double Strand Break Ends". Journal of Biological Chemistry. 280 (46): 38631–38638. doi:10.1074/jbc.M508339200.
  5. ^ Lengsfeld, Bettina M.; Rattray, Alison J.; Bhaskara, Venugopal; Ghirlando, Rodolfo; Paull, Tanya T. (November 2007). "Sae2 Is an Endonuclease that Processes Hairpin DNA Cooperatively with the Mre11/Rad50/Xrs2 Complex". Molecular Cell. 28 (4): 638–651. doi:10.1016/j.molcel.2007.11.001. PMC 2194599.
  6. ^ Cannavo, Elda; Cejka, Petr (October 2014). "Sae2 promotes dsDNA endonuclease activity within Mre11–Rad50–Xrs2 to resect DNA breaks". Nature. 514 (7520): 122–125. doi:10.1038/nature13771.
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