Transcription initiation protein SPT3 homolog is a protein that in humans is encoded by the SUPT3H gene .[5] [6] [7]
Interactions [ ] Transcription initiation protein SPT3 homolog has been shown to interact with GCN5L2 ,[8] [9] TAF6L ,[9] TADA3L ,[9] TAF5L ,[9] SF3B3 ,[9] SUPT7L ,[9] Myc ,[10] TAF9 ,[9] Transformation/transcription domain-associated protein ,[9] TAF12 ,[9] TAF10 ,[8] [9] TAF4 [8] DDB1 .[9]
Model organisms [ ] Model organisms have been used in the study of SUPT3H function. A conditional knockout mouse line called Supt3tm1a(EUCOMM)Hmgu was generated at the Wellcome Trust Sanger Institute .[11] phenotypic screen [12] [13] [14] [15] [16] [17]
Supt3 knockout mouse phenotype
Characteristic
Phenotype
All data available at.[12] [17]
Peripheral blood leukocytes 6 Weeks
Normal
Insulin
Normal
Haematology Normal
Homozygous viability at P14
Abnormal
Recessive lethal studyAbnormal
Body weight
Normal
Neurological assessment
Normal
Grip strength
Normal
Dysmorphology Normal
Indirect calorimetry Normal
Glucose tolerance test Normal
Auditory brainstem response Normal
DEXA Normal
Radiography Normal
Eye morphology
Normal
Clinical chemistry Normal
Haematology Normal
Peripheral blood leukocytes 16 Weeks
Normal
Heart weight
Normal
Salmonella Normal
Cytotoxic T Cell Function
Normal
Spleen Immunophenotyping
Normal
Mesenteric Lymph Node Immunophenotyping
Normal
Bone Marrow Immunophenotyping
Normal
Epidermal Immune Composition
Normal
Trichuris Challenge
Normal
References [ ]
^ a b c GRCh38: Ensembl release 89: ENSG00000196284 - Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000038954 - Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Ogryzko VV, Kotani T, Zhang X, Schiltz RL, Howard T, Yang XJ, Howard BH, Qin J, Nakatani Y (Jul 1998). "Histone-like TAFs within the PCAF histone acetylase complex" . Cell . 94 (1): 35–44. doi :10.1016/S0092-8674(00)81219-2 PMID 9674425 . S2CID 18942972 . ^ Martinez E, Kundu TK, Fu J, Roeder RG (Sep 1998). "A human SPT3-TAFII31-GCN5-L acetylase complex distinct from transcription factor IID" . The Journal of Biological Chemistry . 273 (37): 23781–5. doi :10.1074/jbc.273.37.23781 PMID 9726987 . ^ "Entrez Gene: SUPT3H suppressor of Ty 3 homolog (S. cerevisiae)" .^ a b c Brand M, Moggs JG, Oulad-Abdelghani M, Lejeune F, Dilworth FJ, Stevenin J, Almouzni G, Tora L (Jun 2001). "UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation" . The EMBO Journal . 20 (12): 3187–96. doi :10.1093/emboj/20.12.3187 . PMC 150203 PMID 11406595 . ^ a b c d e f g h i j k Martinez E, Palhan VB, Tjernberg A, Lymar ES, Gamper AM, Kundu TK, Chait BT, Roeder RG (Oct 2001). "Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo" . Molecular and Cellular Biology . 21 (20): 6782–95. doi :10.1128/MCB.21.20.6782-6795.2001 . PMC 99856 PMID 11564863 . ^ Liu X, Tesfai J, Evrard YA, Dent SY, Martinez E (May 2003). "c-Myc transformation domain recruits the human STAGA complex and requires TRRAP and GCN5 acetylase activity for transcription activation" . The Journal of Biological Chemistry . 278 (22): 20405–12. doi :10.1074/jbc.M211795200 . PMC 4031917 PMID 12660246 . ^ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica . 88 : 925–7. doi :10.1111/j.1755-3768.2010.4142.x . S2CID 85911512 . ^ a b "International Mouse Phenotyping Consortium" .^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function" . Nature . 474 (7351): 337–42. doi :10.1038/nature10163 . PMC 3572410 PMID 21677750 . ^ Dolgin E (Jun 2011). "Mouse library set to be knockout" . Nature . 474 (7351): 262–3. doi :10.1038/474262a PMID 21677718 . ^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons" . Cell . 128 (1): 9–13. doi :10.1016/j.cell.2006.12.018 PMID 17218247 . S2CID 18872015 . ^ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes" . Cell . 154 (2): 452–64. doi :10.1016/j.cell.2013.06.022 . PMC 3717207 PMID 23870131 . ^ a b "Infection and Immunity Immunophenotyping (3i) Consortium" .
Further reading [ ]
Yu J, Madison JM, Mundlos S, Winston F, Olsen BR (Oct 1998). "Characterization of a human homologue of the Saccharomyces cerevisiae transcription factor spt3 (SUPT3H)". Genomics . 53 (1): 90–6. doi :10.1006/geno.1998.5500 . PMID 9787080 . Vassilev A, Yamauchi J, Kotani T, Prives C, Avantaggiati ML, Qin J, Nakatani Y (Dec 1998). "The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily" . Molecular Cell . 2 (6): 869–75. doi :10.1016/S1097-2765(00)80301-9 PMID 9885574 . Brand M, Yamamoto K, Staub A, Tora L (Jun 1999). "Identification of TATA-binding protein-free TAFII-containing complex subunits suggests a role in nucleosome acetylation and signal transduction" . The Journal of Biological Chemistry . 274 (26): 18285–9. doi :10.1074/jbc.274.26.18285 PMID 10373431 . Speek M (Mar 2001). "Antisense promoter of human L1 retrotransposon drives transcription of adjacent cellular genes" . Molecular and Cellular Biology . 21 (6): 1973–85. doi :10.1128/MCB.21.6.1973-1985.2001 . PMC 86790 PMID 11238933 . Brand M, Moggs JG, Oulad-Abdelghani M, Lejeune F, Dilworth FJ, Stevenin J, Almouzni G, Tora L (Jun 2001). "UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation" . The EMBO Journal . 20 (12): 3187–96. doi :10.1093/emboj/20.12.3187 . PMC 150203 PMID 11406595 . Martinez E, Palhan VB, Tjernberg A, Lymar ES, Gamper AM, Kundu TK, Chait BT, Roeder RG (Oct 2001). "Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo" . Molecular and Cellular Biology . 21 (20): 6782–95. doi :10.1128/MCB.21.20.6782-6795.2001 . PMC 99856 PMID 11564863 . Cavusoglu N, Brand M, Tora L, Van Dorsselaer A (Feb 2003). "Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis". Proteomics . 3 (2): 217–23. doi :10.1002/pmic.200390030 . PMID 12601814 . S2CID 6035986 . Liu X, Tesfai J, Evrard YA, Dent SY, Martinez E (May 2003). "c-Myc transformation domain recruits the human STAGA complex and requires TRRAP and GCN5 acetylase activity for transcription activation" . The Journal of Biological Chemistry . 278 (22): 20405–12. doi :10.1074/jbc.M211795200 . PMC 4031917 PMID 12660246 .
