KHDRBS3 has been shown to interact with SIAH1.[8][9]
KHDRBS3 interacts with splicing protein Sam68 and oncogene metadherin in prostate cancer cells.[10]
Clinical significance[]
KHDRBS3 (T-STAR) expression has been shown to be increased in prostate cancer tissue compared to the surrounding benign tissue.[10] Expression of KHDRBS3 correlates with mpMRI signal measured through Likert score a system similar to PI-RADS.[10] While still under debate, mpMRI signal correlates with higher Gleason grade and tumour size, in addition to histopathological features associated with clinically aggressive prostate cancer.[11][12] Expression of KHDRBS3 was increased in the failing human myocardium of heart failure patients, here KHDRBS3 protein interacted with several important mRNAs coding for sarcomere components, such as actin gamma 1 (ACTG1), myosin light chain 2 (MYL2), ryanodine receptor 2 (RYR2), troponin I3 (TNNI3), troponin T2 (TNNT2), tropomyosin 1 (TPM1), tropomyosin 2 (TPM2), and titin (TTN).[13]
In prostate cancer cell lines KHDRBS3 appears to be androgen regulated, with a reduction in mRNA expression occurring following addition of synthetic androgen R1881 to cells.[10]
Function[]
KHDRBS3 regulates the alternative mRNA splicing of the sacromere protein titin (TTN), leading to intron retention. Overexpression of KHDRBS3 in induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) increased Ca2+ transient amplitude and resulted in an increase of Fmax.[13]
^Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID16189514. S2CID4427026.
Sugimoto Y, Morita R, Amano K, Shah PU, Pascual-Castroviejo I, Khan S, et al. (August 2001). "T-STAR gene: fine mapping in the candidate region for childhood absence epilepsy on 8q24 and mutational analysis in patients". Epilepsy Research. 46 (2): 139–44. doi:10.1016/S0920-1211(01)00274-1. PMID11463515. S2CID28713407.
Kool J, van Zaane W, van der Eb AJ, Terleth C (November 2001). "Down-regulation of T-STAR, a growth inhibitory protein, after SV40-mediated immortalization". Cell Growth & Differentiation. 12 (11): 535–41. PMID11714634.
Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID16189514. S2CID4427026.