Monoraphidium neglectum

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Monoraphidium neglectum
Scientific classification edit
(unranked): Viridiplantae
Phylum: Chlorophyta
Class: Chlorophyceae
Order: Sphaeropleales
Family: Selenastraceae
Genus: Monoraphidium
Species:
M. neglectum
Binomial name
Monoraphidium neglectum
Heynig & Krienitz

Monoraphidium neglectum is a single-cell green alga of the family Selenastraceae.[1][2] There is interest in using M. neglectum for the production of biofuels because it can accumulate large quantities (up to 33 % of cell dry weight) of triacylglycerides (TAGs).[3]

In 2013, a draft nuclear, mitochondrial, and chloroplast genome was published with an estimated nuclear size of 68 Mb and approximately 16,761 genes.[4] The nuclear genome is likely diploid.[4] The mitochondrial genome of M. neglectum is 96 kb, which is twice as large as and nearly six times larger than the green alga Chlamydomonas reinhardtii, but contains fewer genes.[4][5]

A nuclear transformation technique has been established for M. neglectum using electrotransformation as the method of DNA delivery.[6] The strategy described in this study, including a pretreatment step to weaken the cell wall,[6] might inspire future studies with other microalgae also aiming at stable nuclear genetic transformation using electrotransformation.

The transcriptome of M. neglectum has been sequenced using Illumina HiSeq technology. Using a poly-A fishing and genome-guided approach, the transcriptome was assembled into 20,751 genes (i. e., loci).[7] The dataset encompasses a time-course experiment under conditions of both, high TAG accumulation and subsequent TAG degradation.[7] TAG accumulation and degradation were induced by nitrogen removal (-N conditions) and nitrogen resupply (NR conditions).[7] In total, the transcriptome was sequenced at 12 time points (t0, 7x -N, 4x NR), yielding a comprehensive and high-quality dataset.[7] The raw data (100 nt paired-end reads) has been deposited at SRA under the SRA Study accession number SRP112537.[7] The processed data is available at http://tdbmn.cebitec.uni-bielefeld.de, at which direct gene and BLAST search enable for data mining and to investigate the expression patterns of target genes under autotrophic -N and NR conditions.[7] It is furthermore possible to compare the expression patterns of target genes of M. neglectum with those of other microalgae which were also subjected to -N conditions to induce TAG accumulation.[7]

References[]

  1. ^ Heynig H, Krienitz L (1982). "Monoraphidium neglectum n. sp. sowie einige Bemerkungen zu den Gattungen Monoraphidium, Chlorolobium und Keratococcus (Chlorococcales)". Archiv für Protistenkunde (in German). 125 (1–4): 335–344. doi:10.1016/S0003-9365(82)80032-8.
  2. ^ See the NCBI webpage on Monoraphidium. Data extracted from the "NCBI taxonomy resources". National Center for Biotechnology Information. Retrieved 2017-03-17.
  3. ^ Jaeger D, Pilger C, Hachmeister H, Oberländer E, Wördenweber R, Wichmann J, Mussgnug JH, Huser T, Kruse O (October 2016). "Label-free in vivo analysis of intracellular lipid droplets in the oleaginous microalga Monoraphidium neglectum by coherent Raman scattering microscopy". Scientific Reports. 6: 35340. doi:10.1038/srep35340. PMC 5073319. PMID 27767024.
  4. ^ a b c Bogen C, Al-Dilaimi A, Albersmeier A, Wichmann J, Grundmann M, Rupp O, Lauersen KJ, Blifernez-Klassen O, Kalinowski J, Goesmann A, Mussgnug JH, Kruse O (December 2013). "Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production". BMC Genomics. 14: 926. doi:10.1186/1471-2164-14-926. PMC 3890519. PMID 24373495.
  5. ^ Radakovits R, Jinkerson RE, Fuerstenberg SI, Tae H, Settlage RE, Boore JL, Posewitz MC (February 2012). "Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana". Nature Communications. 3: 686. doi:10.1038/ncomms1688. PMC 3293424. PMID 22353717.
  6. ^ a b Jaeger D, Hübner W, Huser T, Mussgnug JH, Kruse O (May 2017). "Nuclear transformation and functional gene expression in the oleaginous microalga Monoraphidium neglectum". Journal of Biotechnology. 249: 10–15. doi:10.1016/j.jbiotec.2017.03.011. PMID 28302588.
  7. ^ a b c d e f g Jaeger D, Winkler A, Mussgnug JH, Kalinowski J, Goesmann A, Kruse O (2017-08-14). "Monoraphidium neglectum reveal a model for triacylglycerol and lipid hyperaccumulation". Biotechnology for Biofuels. 10: 197. doi:10.1186/s13068-017-0882-1. PMC 5556983. PMID 28814974.

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