OmrA-B RNA

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OmrA-B RNA
OmrA-B RNA structure.png
A representation of the bacterial OmrA-B secondary structure including a colour scheme that indicates the degree of sequence conservation.
Identifiers
SymbolOmrA-B
Alt. SymbolsOmrA, OmrB, SraE, RygA, RygB
RfamRF00079
Other data
RNA typeGene
Domain(s)Bacteria; Enterobacteriaceae
PDB structuresPDBe

The OmrA-B RNA gene family (also known as SraE RNA, RygA and RygB and OmrA and OmrB) is a pair of homologous -regulated small non-coding RNA that was discovered in E. coli during two large-scale screens.[1][2] OmrA-B is highly abundant in stationary phase, but low levels could be detected in exponentially growing cells as well. RygB is adjacent to RygA a closely related RNA. These RNAs bind to the Hfq protein and regulate gene expression by antisense binding. They negatively regulate the expression of several genes encoding outer membrane proteins, including , CsgD, , fepA and ompT by binding in the vicinity of the Shine-Dalgarno sequence, suggesting the control of these targets is dependent on Hfq protein and RNase E. Taken together, these data suggest that OmrA-B participates in the regulation of outer membrane composition, responding to environmental conditions.[3][4][5]

Together with the RNA chaperone Hfq, OmrA-B positively controls bacterial motility and negatively controls the production of acidic exopolysaccharide amylovoran in plant pathogen Erwinia amylovora.[6]

References[]

  1. ^ Argaman, L; Hershberg R; Vogel J; Bejerano G; Wagner EG; Margalit H; Altuvia S (2001). "Novel small RNA-encoding genes in the intergenic regions of Escherichia coli". Curr Biol. 11 (12): 941–950. doi:10.1016/S0960-9822(01)00270-6. PMID 11448770.
  2. ^ Wassarman, KM; Repoila F; Rosenow C; Storz G; Gottesman S (2001). "Identification of novel small RNAs using comparative genomics and microarrays". Genes Dev. 15 (13): 1637–1651. doi:10.1101/gad.901001. PMC 312727. PMID 11445539.
  3. ^ Guillier M, Gottesman S (2006). "Remodelling of the Escherichia coli outer membrane by two small regulatory RNAs". Mol Microbiol. 59 (1): 231–247. doi:10.1111/j.1365-2958.2005.04929.x. PMID 16359331.
  4. ^ Guillier M, Gottesman S (2008). "The 5′ end of two redundant sRNAs is involved in the regulation of multiple targets, including their own regulator". Nucleic Acids Res. 36 (21): 6781–6794. doi:10.1093/nar/gkn742. PMC 2588501. PMID 18953042.
  5. ^ Holmqvist E, Reimegård J, Sterk M, Grantcharova N, Römling U, Wagner EG (2010). "Two antisense RNAs target the transcriptional regulator CsgD to inhibit curli synthesis". EMBO J. 29 (11): 1840–1850. doi:10.1038/emboj.2010.73. PMC 2885931. PMID 20407422.
  6. ^ Zeng, Quan; Sundin, George W. (2014-01-01). "Genome-wide identification of Hfq-regulated small RNAs in the fire blight pathogen Erwinia amylovora discovered small RNAs with virulence regulatory function". BMC Genomics. 15: 414. doi:10.1186/1471-2164-15-414. ISSN 1471-2164. PMC 4070566. PMID 24885615.

External links[]

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