Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands and phosphomonoesters from RNA.[1][2] This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate.[3] This prevents recircularization of the linearized DNA vector and improves the yield of the vector containing the appropriate insert.
References[]
^Sambrook J, Fritsch EF, Maniatis T (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
^Seeburg PH, Shine J, Martial JA, Baxter JD, Goodman HM (December 1977). "Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone". Nature. 270 (5637): 486–94. Bibcode:1977Natur.270..486S. doi:10.1038/270486a0. PMID339105. S2CID4196683.
^Ullrich A, Shine J, Chirgwin J, Pictet R, Tischer E, Rutter WJ, Goodman HM (June 1977). "Rat insulin genes: construction of plasmids containing the coding sequences". Science. New York, N.Y. 196 (4296): 1313–9. Bibcode:1977Sci...196.1313U. doi:10.1126/science.325648. PMID325648.
