Haptoglobin
HP | |||||||||||||
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Aliases | HP, BP, HP2ALPHA2, HPA1S, haptoglobin | ||||||||||||
External IDs | OMIM: 140100 MGI: 96211 HomoloGene: 121756 GeneCards: HP | ||||||||||||
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Species | Human | Mouse | |||||||||||
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Location (UCSC) | Chr 16: 72.05 – 72.06 Mb | Chr 8: 109.58 – 109.58 Mb | |||||||||||
PubMed search | [3] | [4] | |||||||||||
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![](http://upload.wikimedia.org/wikipedia/commons/thumb/b/b5/Hem-hap_4f4o.jpg/220px-Hem-hap_4f4o.jpg)
Haptoglobin (abbreviated as Hp) is the protein that in humans is encoded by the HP gene.[5][6] In blood plasma, haptoglobin binds to free hemoglobin,[7] compared to hemopexin that binds to free heme,[8] released from erythrocytes with high affinity, and thereby inhibits its deleterious oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen).
In clinical settings, the haptoglobin assay is used to screen for and monitor intravascular hemolytic anemia. In intravascular hemolysis, free hemoglobin will be released into circulation and hence haptoglobin will bind the hemoglobin. This causes a decline in haptoglobin levels.
Function[]
Hemoglobin that has been released into the blood plasma by damaged red blood cells has harmful effects. The HP gene encodes a preproprotein that is processed to yield both alpha and beta chains, which subsequently combines as a tetramer to produce haptoglobin. Haptoglobin functions to bind the free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin.[9]
The cellular receptor target of Hp is the monocyte/macrophage scavenger receptor, CD163.[7] Following Hb-Hp binding to CD163, cellular internalization of the complex leads to globin and heme metabolism, which is followed by adaptive changes in antioxidant and iron metabolism pathways and macrophage phenotype polarization.[7]
Differentiation with hemopexin[]
When hemoglobin is released from RBCs within the physiologic range of hemopexin, the potential deleterious effects of hemoglobin are prevented. However, during hyper-hemolytic conditions or with chronic hemolysis, hemoglobin is depleted and readily distributes to tissues where it might be exposed to oxidative conditions. In such conditions, heme can be released from ferric (Fe3+-bound) hemoglobin. The free heme can then accelerate tissue damage by promoting peroxidative reactions and activation of inflammatory cascades. Hemopexin (Hx) is another plasma glycoprotein, like hemoglobin, that is able to bind heme with high affinity. Hemopexin sequesters heme in an inert, non-toxic form and transports it to the liver for catabolism and excretion.[7]
Synthesis[]
Haptoglobin is produced mostly by hepatic cells but also by other tissues such as skin, lung and kidney. In addition, the haptoglobin gene is expressed in murine and human adipose tissue.[10]
Haptoglobin had been shown to be expressed in adipose tissue of cattle as well.[11]
Structure[]
Haptoglobin, in its simplest form, consists of two alpha and two beta chains, connected by disulfide bridges. The chains originate from a common precursor protein, which is proteolytically cleaved during protein synthesis.
Hp exists in two allelic forms in the human population, so-called Hp1 and Hp2, the latter one having arisen due to the partial duplication of Hp1 gene. Three genotypes of Hp, therefore, are found in humans: Hp1-1, Hp2-1, and Hp2-2. Hp of different genotypes have been shown to bind hemoglobin with different affinities, with Hp2-2 being the weakest binder.
In other species[]
Hp has been found in all mammals studied so far, some birds, e.g., cormorant and ostrich but also, in its simpler form, in bony fish, e.g., zebrafish. Hp is absent in at least some amphibians (Xenopus) and neognathous birds (chicken and goose).
Clinical significance[]
Mutations in this gene or its regulatory regions cause or . This gene has also been linked to diabetic nephropathy,[12] the incidence of coronary artery disease in type 1 diabetes,[13] Crohn's disease,[14] inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease,[15] and a reduced incidence of Plasmodium falciparum malaria.[16]
Since the reticuloendothelial system will remove the haptoglobin-hemoglobin complex from the body,[8] haptoglobin levels will be decreased in case of intravascular hemolysis or severe extravascular hemolysis. In the process of binding to free hemoglobin, haptoglobin sequesters the iron within hemoglobin, preventing iron-utilizing bacteria from benefiting from hemolysis. It is theorized that, because of this, haptoglobin has evolved into an acute-phase protein. HP has a protective influence on the hemolytic kidney.[17][18]
The different haptoglobin phenotypes differ in their antioxidant, scavenging, and immunomodulatory properties. This aspect of haptoglobin may gain importance in immune suppressed conditions (such as liver cirrhosis) and the various phenotypes may result in different susceptibility levels towards bacterial infections.[19]
Some studies associate certain haptoglobin phenotypes with the risk of developing schizophrenia.[20]
Test protocol[]
Measuring the level of haptoglobin in a patient's blood is ordered whenever a patient exhibits symptoms of anemia, such as pallor, fatigue, or shortness of breath, along with physical signs of hemolysis, such as jaundice or dark-colored urine. The test is also commonly ordered as a hemolytic anemia battery, which also includes a reticulocyte count and a peripheral blood smear. It can also be ordered along with a direct antiglobulin test when a patient is suspected of having a transfusion reaction or symptoms of autoimmune hemolytic anemia. Also, it may be ordered in conjunction with a bilirubin.
Interpretation[]
A decrease in haptoglobin can support a diagnosis of intravascular hemolytic anemia, especially when correlated with a decreased red blood cell count, hemoglobin, and hematocrit, and also an increased reticulocyte count.
If the reticulocyte count is increased, but the haptoglobin level is normal, this may indicate that cellular destruction is occurring in the spleen and liver, which may indicate an extravascular hemolytic anemia, drug-induced hemolysis, or a red cell dysplasia. The spleen and liver recognize an error in the red cells (either drug coating the red cell membrane or a dysfunctional red cell membrane), and destroy the cell. This type of destruction does not release hemoglobin into the peripheral blood, so the haptoglobin cannot bind to it. Thus, the haptoglobin will stay normal if the hemolysis is not severe. In severe extra-vascular hemolysis, haptoglobin levels can also be low, when large amount of hemoglobin in the reticuloendothelial system leads to transfer of free hemoglobin into plasma.[21]
If there are symptoms of anemia but both the reticulocyte count and the haptoglobin level are normal, the anemia is most likely not due to hemolysis, but instead some other error in cellular production, such as aplastic anemia.
Haptoglobin levels that are decreased but do not accompany signs of anemia may indicate liver damage, as the liver is not producing enough haptoglobin to begin with.
As haptoglobin is indeed an acute-phase protein, any inflammatory process (infection, extreme stress, burns, major crush injury, allergy, etc.) may increase the levels of plasma haptoglobin.
See also[]
- Hemopexin
- Haptoglobin-related protein
References[]
- This article incorporates text from the United States National Library of Medicine, which is in the public domain.
- ^ Jump up to: a b c GRCh38: Ensembl release 89: ENSG00000257017 - Ensembl, May 2017
- ^ Jump up to: a b c GRCm38: Ensembl release 89: ENSMUSG00000031722 - Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Dobryszycka W (September 1997). "Biological functions of haptoglobin--new pieces to an old puzzle". European Journal of Clinical Chemistry and Clinical Biochemistry. 35 (9): 647–54. PMID 9352226.
- ^ Wassell J (2000). "Haptoglobin: function and polymorphism". Clinical Laboratory. 46 (11–12): 547–52. PMID 11109501.
- ^ Jump up to: a b c d Schaer DJ, Vinchi F, Ingoglia G, Tolosano E, Buehler PW (28 October 2014). "Haptoglobin, hemopexin, and related defense pathways-basic science, clinical perspectives, and drug development". Frontiers in Physiology. Frontiers Media SA. 5: 415. doi:10.3389/fphys.2014.00415. PMC 4211382. PMID 25389409.
- ^ Jump up to: a b "Intravascular hemolysis". eClinpath. Retrieved 8 May 2019.
- ^ "Entrez Gene: HP".
- ^ Trayhurn P, Wood IS (September 2004). "Adipokines: inflammation and the pleiotropic role of white adipose tissue". Br. J. Nutr. 92 (3): 347–55. doi:10.1079/BJN20041213. PMID 15469638.
- ^ Saremi B, Al-Dawood A, Winand S, Müller U, Pappritz J, von Soosten D, Rehage J, Dänicke S, Häussler S, Mielenz M, Sauerwein H (May 2012). "Bovine haptoglobin as an adipokine: Serum concentrations and tissue expression in dairy cows receiving a conjugated linoleic acids supplement throughout lactation". Vet Immunol Immunopathol. 146 (3–4): 201–11. doi:10.1016/j.vetimm.2012.03.011. PMID 22498004.
- ^ Asleh R, Levy AP (2005). "In vivo and in vitro studies establishing haptoglobin as a major susceptibility gene for diabetic vascular disease". Vasc Health Risk Manag. 1 (1): 19–28. doi:10.2147/vhrm.1.1.19.58930. PMC 1993923. PMID 17319095.
- ^ Sadrzadeh SM, Bozorgmehr J (June 2004). "Haptoglobin phenotypes in health and disorders". Am. J. Clin. Pathol. 121 Suppl: S97–104. doi:10.1309/8GLX5798Y5XHQ0VW. PMID 15298155.
- ^ Papp M, Lakatos PL, Palatka K, Foldi I, Udvardy M, Harsfalvi J, Tornai I, Vitalis Z, Dinya T, Kovacs A, Molnar T, Demeter P, Papp J, Lakatos L, Altorjay I (May 2007). "Haptoglobin polymorphisms are associated with Crohn's disease, disease behavior, and extraintestinal manifestations in Hungarian patients". Dig. Dis. Sci. 52 (5): 1279–84. doi:10.1007/s10620-006-9615-1. PMID 17357835. S2CID 35999438.
- ^ Costa-Mallen P, Checkoway H, Zabeti A, Edenfield MJ, Swanson PD, Longstreth WT, Franklin GM, Smith-Weller T, Sadrzadeh SM (March 2008). "The functional polymorphism of the hemoglobin-binding protein haptoglobin influences susceptibility to idiopathic Parkinson's disease". American Journal of Medical Genetics. 147B (2): 216–22. doi:10.1002/ajmg.b.30593. PMID 17918239. S2CID 21568460.
- ^ Prentice AM, Ghattas H, Doherty C, Cox SE (December 2007). "Iron metabolism and malaria". Food Nutr Bull. 28 (4 Suppl): S524–39. doi:10.1177/15648265070284S406. PMID 18297891.
- ^ Pintera J (1968). "The protective influence of haptoglobin on hemoglobinuric kidney. I. Biochemical and macroscopic observations". Folia Haematol. Int. Mag. Klin. Morphol. Blutforsch. 90 (1): 82–91. PMID 4176393.
- ^ Miederer SE, Hotz J (December 1969). "[Pathogenesis of kidney hemolysis]". Bruns Beitr Klin Chir (in German). 217 (7): 661–5. PMID 5404273.
- ^ Vitalis, Zsuzsanna; Altorjay, Istvan; Tornai, Istvan; Palatka, Karoly; Kacska, Sandor; Palyu, Eszter; Tornai, David; Udvardy, Miklos; Harsfalvi, Jolan; Dinya, Tamas; Veres, Gabor; Lakatos, Peter Laszlo; Papp, Maria (1 April 2011). "Phenotypic polymorphism of haptoglobin: A novel risk factor for the development of infection in liver cirrhosis". Human Immunology. 72 (4): 348–354. doi:10.1016/j.humimm.2011.01.008. ISSN 0198-8859. PMID 21262313.
- ^ Gene Overview of All Published Schizophrenia-Association Studies for HP Archived 21 February 2009 at the Wayback Machine - SzGene database at Schizophrenia Research Forum.
- ^ Temple, Victor. "HEMOLYSIS AND JAUNDICE: An overview" (PDF). Retrieved 9 July 2011.
Further reading[]
- Graversen JH, Madsen M, Moestrup SK (2002). "CD163: a signal receptor scavenging haptoglobin-hemoglobin complexes from plasma". Int. J. Biochem. Cell Biol. 34 (4): 309–14. doi:10.1016/S1357-2725(01)00144-3. PMID 11854028.
- Madsen M, Graversen JH, Moestrup SK (2002). "Haptoglobin and CD163: captor and receptor gating hemoglobin to macrophage lysosomes". Redox Rep. 6 (6): 386–8. doi:10.1179/135100001101536490. PMID 11865982.
- Erickson LM, Kim HS, Maeda N (1993). "Junctions between genes in the haptoglobin gene cluster of primates". Genomics. 14 (4): 948–58. doi:10.1016/S0888-7543(05)80116-8. PMID 1478675.
- Maeda N (1985). "Nucleotide sequence of the haptoglobin and haptoglobin-related gene pair. The haptoglobin-related gene contains a retrovirus-like element". J. Biol. Chem. 260 (11): 6698–709. doi:10.1016/S0021-9258(18)88836-6. PMID 2987228.
- Simmers RN, Stupans I, Sutherland GR (1986). "Localization of the human haptoglobin genes distal to the fragile site at 16q22 using in situ hybridization". Cytogenet. Cell Genet. 41 (1): 38–41. doi:10.1159/000132193. PMID 3455911.
- van der Straten A, Falque JC, Loriau R, Bollen A, Cabezón T (1986). "Expression of cloned human haptoglobin and alpha 1-antitrypsin complementary DNAs in Saccharomyces cerevisiae". DNA. 5 (2): 129–36. doi:10.1089/dna.1986.5.129. PMID 3519135.
- Bensi G, Raugei G, Klefenz H, Cortese R (1985). "Structure and expression of the human haptoglobin locus". EMBO J. 4 (1): 119–26. doi:10.1002/j.1460-2075.1985.tb02325.x. PMC 554159. PMID 4018023.
- Malchy B, Dixon GH (1973). "Studies on the interchain disulfides of human haptoglobins". Can. J. Biochem. 51 (3): 249–64. doi:10.1139/o73-032. PMID 4573324.
- Raugei G, Bensi G, Colantuoni V, Romano V, Santoro C, Costanzo F, Cortese R (1983). "Sequence of human haptoglobin cDNA: evidence that the alpha and beta subunits are coded by the same mRNA". Nucleic Acids Res. 11 (17): 5811–9. doi:10.1093/nar/11.17.5811. PMC 326319. PMID 6310515.
- Yang F, Brune JL, Baldwin WD, Barnett DR, Bowman BH (1983). "Identification and characterization of human haptoglobin cDNA". Proc. Natl. Acad. Sci. U.S.A. 80 (19): 5875–9. Bibcode:1983PNAS...80.5875Y. doi:10.1073/pnas.80.19.5875. PMC 390178. PMID 6310599.
- Maeda N, Yang F, Barnett DR, Bowman BH, Smithies O (1984). "Duplication within the haptoglobin Hp2 gene". Nature. 309 (5964): 131–5. Bibcode:1984Natur.309..131M. doi:10.1038/309131a0. PMID 6325933. S2CID 4368535.
- Brune JL, Yang F, Barnett DR, Bowman BH (1984). "Evolution of haptoglobin: comparison of complementary DNA encoding Hp alpha 1S and Hp alpha 2FS". Nucleic Acids Res. 12 (11): 4531–8. doi:10.1093/nar/12.11.4531. PMC 318856. PMID 6330675.
- van der Straten A, Herzog A, Cabezón T, Bollen A (1984). "Characterization of human haptoglobin cDNAs coding for alpha 2FS beta and alpha 1S beta variants". FEBS Lett. 168 (1): 103–7. doi:10.1016/0014-5793(84)80215-X. PMID 6546723. S2CID 85082741.
- vander Straten A, Herzog A, Jacobs P, Cabezón T, Bollen A (1984). "Molecular cloning of human haptoglobin cDNA: evidence for a single mRNA coding for alpha 2 and beta chains". EMBO J. 2 (6): 1003–7. doi:10.1002/j.1460-2075.1983.tb01534.x. PMC 555221. PMID 6688992.
- Kurosky A, Barnett DR, Lee TH, Touchstone B, Hay RE, Arnott MS, Bowman BH, Fitch WM (1980). "Covalent structure of human haptoglobin: a serine protease homolog". Proc. Natl. Acad. Sci. U.S.A. 77 (6): 3388–92. Bibcode:1980PNAS...77.3388K. doi:10.1073/pnas.77.6.3388. PMC 349621. PMID 6997877.
- Eaton JW, Brandt P, Mahoney JR, Lee JT (1982). "Haptoglobin: a natural bacteriostat". Science. 215 (4533): 691–3. Bibcode:1982Sci...215..691E. doi:10.1126/science.7036344. PMID 7036344.
- Kazim AL, Atassi MZ (1980). "Haemoglobin binding with haptoglobin. Unequivocal demonstration that the beta-chains of human haemoglobin bind to haptoglobin". Biochem. J. 185 (1): 285–7. doi:10.1042/bj1850285. PMC 1161299. PMID 7378053.
- Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba T, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Bento Soares M, Tan F, Thierry-Meg J, Trevaskis E, Underwood K, Wohldman P, Waterston R, Wilson R, Marra M (1997). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807–28. doi:10.1101/gr.6.9.807. PMID 8889549.
- Tabak S, Lev A, Valansi C, Aker O, Shalitin C (1997). "Transcriptionally active haptoglobin-related (Hpr) gene in hepatoma G2 and leukemia molt-4 cells". DNA Cell Biol. 15 (11): 1001–7. doi:10.1089/dna.1996.15.1001. PMID 8945641.
- Koda Y, Soejima M, Yoshioka N, Kimura H (1998). "The haptoglobin-gene deletion responsible for anhaptoglobinemia". American Journal of Human Genetics. 62 (2): 245–52. doi:10.1086/301701. PMC 1376878. PMID 9463309.
External links[]
- Haptoglobins at the US National Library of Medicine Medical Subject Headings (MeSH)
- Overview of all the structural information available in the PDB for UniProt: P00738 (Haptoglobin) at the PDBe-KB.
- Genes on human chromosome 16
- Blood proteins