Nucleic acid methods

Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.

Purification[]

DNA extraction
Phenol–chloroform extraction
Minicolumn purification
RNA extraction
Boom method
Synchronous coefficient of drag alteration (SCODA) DNA purification

Quantification[]

Abundance in weight: spectroscopic nucleic acid quantitation
Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)
High-throughput relative abundance: DNA microarray
High-throughput absolute abundance: serial analysis of gene expression (SAGE)
Size: gel electrophoresis

Synthesis[]

De novo: oligonucleotide synthesis
Amplification: polymerase chain reaction (PCR)

Kinetics[]

Gene function[]

RNA interference^[5]

Other[]

Bisulfite sequencing
DNA sequencing
Expression cloning
Fluorescence in situ hybridization
Lab-on-a-chip
Comparison of nucleic acid simulation software
Northern blot
Nuclear run-on assay
Radioactivity in the life sciences
Southern blot
Differential centrifugation (sucrose gradient)
Toeprinting assay
Several bioinformatics methods, as seen in list of RNA structure prediction software

DNA Extraction Kits[]

There are commercially available DNA extraction kits for variety of biological samples, one such kit is the Taqgen® Blood Genomic DNA Kit, which is suitable for Genomic DNA Extraction from whole Blood, body fluids, buccal swabs, buffy coat and cultured cells. It is a spin column based kit, provide an easy, rapid and efficient purification of high quality genomic DNA. The purified DNA can be used directly in a variety of downstream applications, including PCR, Real-time PCR, southern blotting and restriction enzyme digestion. The kit eliminates the need for expensive resin, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yield purified DNA greater than 30 Kb in size.

https://en.wikipedia.org/wiki/DNA_extraction

How to extract DNA from Dried blood card?[]

Taqgen® Tissue DNA Kit 20 Preps [[1]]

References[]

^ Tang, Wei; Hu, Shichao; Wang, Huaming; Zhao, Yan; Li, Na; Liu, Feng (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations". Chem. Commun. 50 (92): 14352–14355. doi:10.1039/C4CC07041K. PMID 25295484.
^ Ihalainen, Petri; Pettersson, Fredrik; Pesonen, Markus; Viitala, Tapani; Määttänen, Anni; Österbacka, Ronald; Peltonen, Jouko (7 March 2014). "An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes". Nanotechnology. 25 (9): 094009. Bibcode:2014Nanot..25i4009I. doi:10.1088/0957-4484/25/9/094009. PMID 24522116.
^ Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics. 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.
^ Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques. 19 (3): 151–158. PMC 2563918. PMID 19137101.
^ Hannon, Gregory J. (July 2002). "RNA interference". Nature. 418 (6894): 244–251. Bibcode:2002Natur.418..244H. doi:10.1038/418244a. ISSN 1476-4687. PMID 12110901. S2CID 4426281.

External links[]

Protocols for Recombinant DNA Isolation, Cloning, and Sequencing

[1] Tang, Wei; Hu, Shichao; Wang, Huaming; Zhao, Yan; Li, Na; Liu, Feng (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations". Chem. Commun. 50 (92): 14352–14355. doi:10.1039/C4CC07041K. PMID 25295484.

[2] Ihalainen, Petri; Pettersson, Fredrik; Pesonen, Markus; Viitala, Tapani; Määttänen, Anni; Österbacka, Ronald; Peltonen, Jouko (7 March 2014). "An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes". Nanotechnology. 25 (9): 094009. Bibcode:2014Nanot..25i4009I. doi:10.1088/0957-4484/25/9/094009. PMID 24522116.

[3] Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics. 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.

[4] Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques. 19 (3): 151–158. PMC 2563918. PMID 19137101.

[5] Hannon, Gregory J. (July 2002). "RNA interference". Nature. 418 (6894): 244–251. Bibcode:2002Natur.418..244H. doi:10.1038/418244a. ISSN 1476-4687. PMID 12110901. S2CID 4426281.

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