Aerobactin synthase

In enzymology, an aerobactin synthase (EC 6.3.2.39) is an enzyme that catalyzes the chemical reaction

4 ATP + citrate + 2 N⁶-acetyl-N⁶-hydroxy-L-lysine + 2 H₂O ${\displaystyle \rightleftharpoons }$ 4 ADP + 4 phosphate + aerobactin

The 4 substrates of this enzyme are ATP, citrate, , and H₂O, whereas its 3 products are ADP, phosphate, and aerobactin.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is citrate:N⁶-acetyl-N⁶-hydroxy-L-lysine ligase (ADP-forming). This enzyme is also called citrate:6-N-acetyl-6-N-hydroxy-L-lysine ligase (ADP-forming). This enzyme participates in lysine degradation.

References[]

• Appanna DL, Grundy BJ, Szczepan EW, Viswanatha T (1984). "Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1". Biochim. Biophys. Acta. 801: 437–443. doi:10.1016/0304-4165(84)90150-8.
• Gibson F, Magrath DI (1969). "The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I". Biochim. Biophys. Acta. 192 (2): 175–84. doi:10.1016/0304-4165(69)90353-5. PMID 4313071.
• Maurer PJ, Miller M (1982). "Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine". J. Am. Chem. Soc. 104 (11): 3096–3101. doi:10.1021/ja00375a025.
• de Lorenzo V, Bindereif A, Paw BH, Neilands JB (1986). "Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12". J. Bacteriol. 165 (2): 570–8. doi:10.1128/jb.165.2.570-578.1986. PMC 214457. PMID 2935523.
• Challis GL (2005). "A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases". ChemBioChem. 6 (4): 601–11. doi:10.1002/cbic.200400283. PMID 15719346.