ATP + [L-Asp(4-L-Arg)]n + L-Asp ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
This enzyme requires Mg2+ for activity. All enzymes known to have this activity also catalyze the addition of arginine, i.e. cyanophycin synthase (L-arginine-adding) activity. It is structurally similar to Muramyl ligases.
References[]
^Aboulmagd E, Oppermann-Sanio FB, Steinbüchel A (November 2000). "Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308". Archives of Microbiology. 174 (5): 297–306. doi:10.1007/s002030000206. PMID11131019.
^Ziegler K, Deutzmann R, Lockau W (2002). "Cyanophycin synthetase-like enzymes of non-cyanobacterial eubacteria: characterization of the polymer produced by a recombinant synthetase of Desulfitobacterium hafniense". Zeitschrift für Naturforschung C. 57 (5–6): 522–9. doi:10.1515/znc-2002-5-621. PMID12132696.
^Ziegler K, Diener A, Herpin C, Richter R, Deutzmann R, Lockau W (May 1998). "Molecular characterization of cyanophycin synthetase, the enzyme catalyzing the biosynthesis of the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartate (cyanophycin)". European Journal of Biochemistry. 254 (1): 154–9. doi:10.1046/j.1432-1327.1998.2540154.x. PMID9652408.