Cyanophycinase (EC 3.4.15.6 , cyanophycin degrading enzyme , beta-Asp-Arg hydrolysing enzyme , CGPase , CphB , CphE , cyanophycin granule polypeptidase , extracellular CGPase ) is an enzyme .[1] [2] [3] It catalyses the following chemical reaction
[L-Asp(4-L-Arg)]n + H2 O
⇌
{\displaystyle \rightleftharpoons }
[L-Asp(4-L-Arg)]n-1 + L-Asp(4-L-Arg)
The enzyme is highly specific for the branched polypeptide cyanophycin . It is similar to Dipeptidase E , another S51 family serine protease .
References [ ]
^ Obst M, Krug A, Luftmann H, Steinbüchel A (July 2005). "Degradation of cyanophycin by Sedimentibacter hongkongensis strain KI and Citrobacter amalonaticus strain G Isolated from an anaerobic bacterial consortium" . Applied and Environmental Microbiology . 71 (7): 3642–52. doi :10.1128/aem.71.7.3642-3652.2005 . PMC 1169039 . PMID 16000772 .
^ Obst M, Oppermann-Sanio FB, Luftmann H, Steinbüchel A (July 2002). "Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI. The cphE gene from P. anguilliseptica BI encodes a cyanophycinhydrolyzing enzyme" . The Journal of Biological Chemistry . 277 (28): 25096–105. doi :10.1074/jbc.m112267200 . PMID 11986309 .
^ Richter R, Hejazi M, Kraft R, Ziegler K, Lockau W (July 1999). "Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin): molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme" . European Journal of Biochemistry . 263 (1): 163–9. doi :10.1046/j.1432-1327.1999.00479.x . PMID 10429200 .
External links [ ]
3.4.11 -19: Exopeptidase
3.4.11 3.4.13 3.4.14 3.4.15 3.4.16 3.4.17 Other/ungrouped
3.4.21 -25: Endopeptidase
Other/ungrouped: Amyloid precursor protein secretase
3.4.99 : Unknown
Activity Regulation Classification Kinetics Types