ERG5

From Wikipedia, the free encyclopedia
Sterol 22-desaturase
Identifiers
OrganismSaccharomyces cerevisiae S288c
SymbolERG5
Alt. symbolsCYP61A1
Entrez855029
HomoloGene20212
RefSeq (mRNA)NM_001182511.1
RefSeq (Prot)NP_013728.1
UniProtP54781
Other data
EC number1.14.19.41
ChromosomeXIII: 0.3 - 0.3 Mb

ERG5 or Sterol 22-desaturase is a cytochrome P450 enzyme in the ergosterol biosynthesis pathway of fungi Saccharomyces cerevisiae (Baker's yeast, a model organism), with the CYP Symbol CYP61A1.[1] CYP61A1 is one of only three P450 enzyme found in baker's yeast, the other two are CYP51F1 and CYP56A1.[2] The ortholog in Schizosaccharomyces pombe (fission yeast, the second sequenced model fungi), was named CYP61A3 for historical reasons, and is only one of two P450 enzyme found with CYP51F1.[3] ERG5 catalyzes the C22-C23 double bond formation on the sterol side chain of ergostatrienol to convert it into , then the C24 double bond of ergostatetrenol will be hydrogenation reduced into ergosterol by ERG4.[4]

Ortholog[]

Most fungi have only one CYP61 gene, and CYP61 global distributed in fungi.[5][6] There are some of the ortholog found in early genome sequenced organism:[7]

CYP symbol Species UniProt/EMBL
CYP61A1 Saccharomyces cerevisiae P54781
CYP61A2 Candida albicans A0A1B3B2L4
CYP61A3 Schizosaccharomyces pombe O13820
CYP61A4 Botrytis cinerea AL111744
CYP61A5 Neurospora crassa

References[]

  1. ^ Ghaemmaghami S, Huh WK, Bower K, Howson RW, Belle A, Dephoure N, et al. (October 2003). "Global analysis of protein expression in yeast". Nature. 425 (6959): 737–41. Bibcode:2003Natur.425..737G. doi:10.1038/nature02046. PMID 14562106. S2CID 4344864.
  2. ^ Nelson DR (January 2018). "Cytochrome P450 diversity in the tree of life". Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1866 (1): 141–154. doi:10.1016/j.bbapap.2017.05.003. PMC 5681887. PMID 28502748.
  3. ^ Wilhelm BT, Marguerat S, Watt S, Schubert F, Wood V, Goodhead I, et al. (June 2008). "Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution". Nature. 453 (7199): 1239–43. Bibcode:2008Natur.453.1239W. doi:10.1038/nature07002. PMID 18488015. S2CID 205213499.
  4. ^ Kelly SL, Lamb DC, Baldwin BC, Corran AJ, Kelly DE (April 1997). "Characterization of Saccharomyces cerevisiae CYP61, sterol delta22-desaturase, and inhibition by azole antifungal agents". The Journal of Biological Chemistry. 272 (15): 9986–8. doi:10.1074/JBC.272.15.9986. PMID 9092539.
  5. ^ Chen W, Lee MK, Jefcoate C, Kim SC, Chen F, Yu JH (June 2014). "Fungal cytochrome p450 monooxygenases: their distribution, structure, functions, family expansion, and evolutionary origin". Genome Biology and Evolution. 6 (7): 1620–34. doi:10.1093/gbe/evu132. PMC 4122930. PMID 24966179.
  6. ^ Moktali V, Park J, Fedorova-Abrams ND, Park B, Choi J, Lee YH, Kang S (October 2012). "Systematic and searchable classification of cytochrome P450 proteins encoded by fungal and oomycete genomes". BMC Genomics. 13: 525. doi:10.1186/1471-2164-13-525. PMC 3505482. PMID 23033934.
  7. ^ Nelson DR (25 March 1999). "Note on P450 evolution in yeasts and early eukaryotes". The Cytochrome P450 Homepage. The University of Tennessee Health Science Center. doi:10.1186/1479-7364-4-1-59. Archived from the original on 25 October 2015.


Stub icon

This enzyme-related article is a stub. You can help Wikipedia by .

  • v
  • t
Retrieved from ""