Ent-kaurene oxidase

From Wikipedia, the free encyclopedia
Ent-kaurene oxidase
Identifiers
EC no.1.14.14.86
CAS no.149565-67-3
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

Ent-kaurene oxidase (EC 1.14.14.86, Formerly EC 1.14.13.78) is an enzyme with systematic name ent-kaur-16-ene,NADPH:oxygen oxidoreductase (hydroxylating).[1][2][3] This enzyme catalyses the following chemical reaction

ent-kaur-16-ene + 3 NADPH + 3 H+ + 3 O2 ent-kaur-16-en-19-oate + 3 NADP+ + 4 H2O (overall reaction)
(1a) ent-kaur-16-ene + NADPH + H+ + O2 ent-kaur-16-en-19-ol + NADP+ + H2O
(1b) ent-kaur-16-en-19-ol + NADPH + H+ + O2 ent-kaur-16-en-19-al + NADP+ + 2 H2O
(1c) ent-kaur-16-en-19-al + NADPH + O2 ent-kaur-16-en-19-oate + NADP+ + H2O

Ent-kaurene oxidase requires cytochrome P450.

References[]

  1. ^ Ashman PJ, Mackenzie A, Bramley PM (November 1990). "Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi". Biochimica et Biophysica Acta (BBA) - General Subjects. 1036 (2): 151–7. doi:10.1016/0304-4165(90)90027-t. PMID 2223832.
  2. ^ Archer C, Ashman PJ, Hedden P, Bowyer JR, Bramley PM (May 1992). "Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima". Biochemical Society Transactions. 20 (2): 218S. doi:10.1042/bst020218s. PMID 1397591.
  3. ^ Helliwell CA, Poole A, Peacock WJ, Dennis ES (February 1999). "Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis". Plant Physiology. 119 (2): 507–10. doi:10.1104/pp.119.2.507. PMC 32127. PMID 9952446.

External links[]


Stub icon

This enzyme-related article is a stub. You can help Wikipedia by .

  • v
  • t
Retrieved from ""