List of restriction enzyme cutting sites: Bst–Bv

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Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

This article contains a list of the most studied restriction enzymes whose names start with Bst to Bv inclusive. It contains approximately 200 enzymes.

The following information is given:

  • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
  • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme:  EcoR70I 


Whole list navigation[]

Restriction enzymes[]

Bst[]

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
Bacillus stearothermophilus 1503-4R 5' GGATCC
3' CCTAGG
5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'
AccEBI, AliI, ApaCI, AsiI, BamHI, BnaI, BspAAIII, Nsp29132II
Bacillus stearothermophilus 1 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AglI, BseBI, Bse17I, BstNI, CbrI, CthII, EcoRII, MvaI, SspAI, TaqXI
Bacillus stearothermophilus 2 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AglI, BseBI, Bse17I, BstNI, BstOI, Bst2UI, CthII, EcoRII, MvaI
Bacillus stearothermophilus 6 5' CTCTTC
3' GAGAAG
5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
Bacillus stearothermophilus 11 5' ACTGG
3' TGACC
5' ---ACTGGN   --- 3'
3' ---TGAC   CN--- 5'
Bacillus stearothermophilus 12 5' GCAGC
3' CGTCG
5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5'
AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst71I, BstV1I
Bacillus stearothermophilus 19 5' GCATC
3' CGTAG
5' ---GCATCNNNN   NN--- 3'
3' ---CGTAGNNNNNN   --- 5'
Bacillus stearothermophilus 19 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAI
Bacillus stearothermophilus 28 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
BciBI, BdiI, Bli41I, Bsa29I, Bsp106I, BspDI, LplI, SpmI, ZhoI
Bacillus stearothermophilus 38 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AorI, BseBI, Bse17I, BsiLI, BspNI, BstOI, Bst2UI, EcoRII, MvaI
Bacillus stearothermophilus 40 5' CCGG
3' GGCC
5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
Bacillus stearothermophilus 71 5' GCAGC
3' CGTCG
5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5'
AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, BstV1I
Bacillus stearothermophilus C98 5' CTTAAG
3' GAATTC
5' ---C   TTAAG--- 3'
3' ---GAATT   C--- 5'
Bacillus stearothermophilus 100 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaI, SleI
Bacillus stearothermophilus RFL1107 5' GTATAC
3' CATATG
5' ---GTA   TAC--- 3'
3' ---CAT   ATG--- 5'
Bacillus stearothermophilus AC 5' GRCGYC
3' CYGCRG
5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5'
AcyI, AhaII, AosII, AstWI, AsuIII, BsaHI, HgiI, HgiDI, Hsp92I
Bacillus stearothermophilus AP 5' GCAN4NTGC
3' CGTN4NACG
5' ---GCANNNN   NTGC--- 3'
3' ---CGTN   NNNNACG--- 5'
ApaBI
Bacillus stearothermophilus AU 5' TGTACA
3' ACATGT
5' ---T   GTACA--- 3'
3' ---ACATG   T--- 5'
AauI,  BsmRI,  Bsp1407I, BsrGI,
Ssp4800I, SspBI
Bacillus stearothermophilus B225 5' TTCGAA
3' AAGCTT
5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5'
AcpI, Bim19I, Bpu14I, BspT104I, Csp45I, LspI, NspV, SspRFI
Bacillus stearothermophilus 2B 5' CACGAG
3' GTGCTC
5' ---C   ACGAG--- 3'
3' ---GTGCT   C--- 5'
Bacillus stearothermophilus BA 5' YACGTR
3' RTGCAY
5' ---YAC   GTR--- 3'
3' ---RTG   CAY--- 5'
Bacillus stearothermophilus BS 5' GTATAC
3' CATATG
5' ---GTA   TAC--- 3'
3' ---CAT   ATG--- 5'
Bacillus stearothermophilus B7S 5' RCCGGY
3' YGGCCR
5' ---R   CCGGY--- 3'
3' ---YGGCC   R--- 5'
Bacillus stearothermophilus BS32 5' GAAGAC
3' CTTCTG
5' ---GAAGACNN   NNNN--- 3'
3' ---CTTCTGNNNNNN   --- 5'
Bacillus stearothermophilus BZ153 5' GCGCGC
3' CGCGCG
5' ---G   CGCGC--- 3'
3' ---CGCGC   G--- 5'
Bacillus stearothermophilus 4C 5' ACNGT
3' TGNCA
5' ---ACN   GT--- 3'
3' ---TG   NCA--- 5'
Bacillus stearothermophilus C8 5' GCNNGC
3' CGNNCG
5' ---GCN   NGC--- 3'
3' ---CGN   NCG--- 5'
Bacillus stearothermophilus D102 5' CCGCTC
3' GGCGAG
5' ---CCG   CTC--- 3'
3' ---GGC   GAG--- 5'
AccBSI, BsrBI, Bst31NI, MbiI
Bacillus stearothermophilus DE 5' CTNAG
3' GANTC
5' ---C   TNAG--- 3'
3' ---GANT   C--- 5'
Bacillus stearothermophilus DS 5' CCRYGG
3' GGYRCC
5' ---C   CRYGG--- 3'
3' ---GGYRC   C--- 5'
Bacillus stearothermophilus ET 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
AcrII, BstPI, BstT9I, BstT10I, EcaI, Eco91I, EcoO65I, NspSAII, PspEI
Bacillus stearothermophilus EN 5' CCTN4NAGG
3' GGAN4NTCC
5' ---CCTNN   NNNAGG--- 3'
3' ---GGANNN   NNTCC--- 5'
Bacillus stearothermophilus EN 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAI
Bacillus stearothermophilus EZ359 5' GTTAAC
3' CAATTG
5' ---GTT   AAC--- 3'
3' ---CAA   TTG--- 5'
Bacillus stearothermophilus FH58 5' AAGCTT
3' TTCGAA
5' ---A   AGCTT--- 3'
3' ---TTCGA   A--- 5'
Bacillus stearothermophilus F5 5' GGATG
3' CCTAC
5' ---GGATGNN   --- 3'
3' ---CCTAC   NN--- 5'
Bacillus stearothermophilus 5' CGCG
3' GCGC
5' ---CG   CG--- 3'
3' ---GC   GC--- 5'
AccII, BceBI, BepI, Bpu95I, BstUI, Csp68KVI, FauBII, MvnI, SelI
Bacillus stearothermophilus FZ438 5' CCCGC
3' GGGCG
5' ---CCCGCNNNN   NN--- 3'
3' ---GGGCGNNNNNN   --- 5'
Bacillus stearothermophilus GZ53 5' CGTCTC
3' GCAGAG
5' ---CGTCTCN   NNNN--- 3'
3' ---GCAGAGNNNNN   --- 5'
Bacillus stearothermophilus H2 5' RGCGCY
3' YCGCGR
5' ---RGCGC   Y--- 3'
3' ---Y   CGCGR--- 5'
AccB2I,  BfoI,  Bme142I,
Bsp143II, HaeII, LpnI
Bacillus stearothermophilus H9 5' GGATC
3' CCTAG
5' ---GGATCNNNN   N--- 3'
3' ---CCTAGNNNNN   --- 5'
AclWI, AlwI, BinI,  BpuFI,  BspPI,
 Bst31TI, BthII, Bth617I,  EacI
Bacillus stearothermophilus HH 5' GCGC
3' CGCG
5' ---GCG   C--- 3'
3' ---C   GCG--- 5'
AspLEI, BspLAI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI
Bacillus stearothermophilus HP 5' GTTAAC
3' CAATTG
5' ---GTT   AAC--- 3'
3' ---CAA   TTG--- 5'
Bacillus stearothermophilus HZ55 5' CCAN6TGG
3' GGTN6ACC
5' ---CCANNNNN   NTGG--- 3'
3' ---GGTN   NNNNNACC--- 5'
Bacillus stearothermophilus IZ316 5' CACNNNGTG
3' GTGNNNCAC
5' ---CACNNN   GTG--- 3'
3' ---GTG   NNNCAC--- 5'
AdeI, DraIII
Bacillus stearothermophilus JZ301 5' CTNAG
3' GANTC
5' ---C   TNAG--- 3'
3' ---GANT   C--- 5'
Bacillus stearothermophilus KT 5' GATC
3' CTAG
5' ---GAT   C--- 3'
3' ---C   TAG--- 5'
AspMDI, Bsp2095I, Bst19II, BspKT6I, DpnII, MboI, NmeCI, SsiAI
Bacillus stearothermophilus M6 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, Fsp1604I, MvaI
Bacillus stearothermophilus MA 5' GTCTC
3' CAGAG
5' ---GTCTCN   NNNNN--- 3'
3' ---CAGAGNNNNN   N--- 5'
Alw26I,  BcoDI, BscQII,  BsmAI,
BsoMAI
Bacillus stearothermophilus MB 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, NdeII, Sth368I
Bacillus stearothermophilus MC 5' CGRYCG
3' GCYRGC
5' ---CGRY   CG--- 3'
3' ---GC   YRGC--- 5'
Bacillus stearothermophilus MW 5' GCN7GC
3' CGN7CG
5' ---GCNNNNN   NNGC--- 3'
3' ---CGNN   NNNNNCG--- 5'
Bacillus stearothermophilus MZ611 5' CCNGG
3' GGNCC
5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
Bacillus stearothermophilus 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AjnI, BciBII, BptI, Bst1I, BstOI, Bst2UI, Fsp1604I, SniI, Sth117I
Bacillus stearothermophilus 31N 5' CCGCTC
3' GGCGAG
5' ---CCG   CTC--- 3'
3' ---GGC   GAG--- 5'
AccBSI, BsrBI, BstD102I, MbiI
Bacillus stearothermophilus 1161NS 5' RCATGY
3' YGTACR
5' ---RCATG   Y--- 3'
3' ---Y   GTACR--- 5'
Bacillus stearothermophilus NZ169 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
BanIII, BciBI, BdiI, Bli41I, Bsa29I, BspDI, LplI, Rme21I, SpmI, ZhoI
Bacillus stearothermophilus O22 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AjnI, ApyI, BciBII, BptI, Bst1I, Bst2UI, Fsp1604I, SniI, Sth117I
Bacillus stearothermophilus OZ616 5' GGGAC
3' CCCTG
5' ---GGGACN8NN   NNNN--- 3'
3' ---CCCTGN8NNNNNN   --- 5'
Bacillus stearothermophilus 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
AcrII, AspAI, BstEII, BstT9I, EcaI, Eco91I, EcoO65I, NspSAII, PspEI
Bacillus stearothermophilus PA 5' GACN4GTC
3' CTGN4CAG
5' ---GACNN   NNGTC--- 3'
3' ---CTGNN   NNCAG--- 5'
5' GGATG
3' CCTAC
5' ---GGATGN8N   NNNN--- 3'
3' ---CCTACN8NNNNN   --- 5'
Bacillus stearothermophilus PZ740 5' CTTAAG
3' GAATTC
5' ---C   TTAAG--- 3'
3' ---GAATT   C--- 5'
Bacillus stearothermophilus RZ246 5' ATTTAAAT
3' TAAATTTA
5' ---ATTT   AAAT--- 3'
3' ---TAAA   TTTA--- 5'
Bacillus stearothermophilus S183 5' CYCGRG
3' GRGCYC
5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5'
Ama87I, AvaI, Bse15I, BsoBI, Eco27kI, NspIII, OfoI, PunAI
Bacillus stearothermophilus SC 5' CCNGG
3' GGNCC
5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
Bacillus stearothermophilus SF 5' CTRYAG
3' GAYRTC
5' ---C   TRYAG--- 3'
3' ---GAYRT   C--- 5'
Bacillus stearothermophilus SN 5' TACGTA
3' ATGCAT
5' ---TAC   GTA--- 3'
3' ---ATG   CAT--- 5'
Bacillus stearothermophilus SW 5' ATTTAAAT
3' TAAATTTA
5' ---ATTT   AAAT--- 3'
3' ---TAAA   TTTA--- 5'
Bacillus stearothermophilus T7 5' TGATCA
3' ACTAGT
5' ---T   GATCA--- 3'
3' ---ACTAG   T--- 5'
AbaI, BclI, BsiQI,
BspXII, FbaI, Ksp22I,  ParI 
Bacillus stearothermophilus T9 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
AspAI, Bse64I, BstT10I, BstPI, Eco91I, EcoO128I, NspSAII, PspEI
Bacillus stearothermophilus T10 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
AspAI, BseT10I, BstEII, BstT10I, Eco91I, EcoO128I, NspSAII, PspEI
Bacillus stearothermophilus 31T 5' GGATC
3' CCTAG
5' ---GGATCNNNN   N--- 3'
3' ---CCTAGNNNNN   --- 5'
Bacillus stearothermophilus TS5 5' GAAGAC
3' CTTCTG
5' ---GAAGACNN   NNNN--- 3'
3' ---CTTCTGNNNNNN   --- 5'
Bacillus stearothermophilus U458 5' CGCG
3' GCGC
5' ---CG   CG--- 3'
3' ---GC   GC--- 5'
AccII, BceBI, BepI, Bpu95I, BtkI, Csp68KVI, FauBII, MvnI, SelI
Bacillus stearothermophilus 2U 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AjnI, BciBII, BptI, Bst1I, BstOI, BstM6I, SniI, SslI, TaqXI, ZanI
Bacillus stearothermophilus V 5' CTCGAG
3' GAGCTC
5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5'
BluI, BssHI, MlaAI, PanI, SauLPII, SciI, SlaI, StrI, XhoI
Bacillus stearothermophilus V1 5' GCAGC
3' CGTCG
5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5'
AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I
Bacillus stearothermophilus V2 5' GAAGAC
3' CTTCTG
5' ---GAAGACNN   NNNN--- 3'
3' ---CTTCTGNNNNNN   --- 5'
Bacillus stearothermophilus X1 5' CCAN6TGG
3' GGTN6ACC
5' ---CCANNNNN   NTGG--- 3'
3' ---GGTN   NNNNNACC--- 5'
Bacillus stearothermophilus X2 5' RGATCY
3' YCTAGR
5' ---R   GATCY--- 3'
3' ---YCTAG   R--- 5'
1SDO Bacillus stearothermophilus Y406 5' RGATCY
3' YCTAGR
5' ---R   GATCY--- 3'
3' ---YCTAG   R--- 5'
Bacillus stearothermophilus Z130 5' CGGCCG
3' GCCGGC
5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5'
AaaI, BseX3I, EagI, EclXI, Eco52I, SenPT16I, XmaIII
Bacillus stearothermophilus 38M 5' GTATAC
3' CATATG
5' ---GTA   TAC--- 3'
3' ---CAT   ATG--- 5'

Bsu - Bv[]

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
Bacillus subtilis 6v1 5' CTCTTC
3' GAGAAG
5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
Bacillus subtilis 15 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
AagI, BanIII, BavCI, Bsa29I, BseCI, BspDI, BsuTUI, ClaI
Bacillus subtilis 23 5' TCCGGA
3' AGGCCT
5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5'
AccIII, BbvAIII, BseAI, BsiMI, BspEI, BspMII, CauB3I, MroI
Bacillus subtilis 36 5' CCTNAGG
3' GGANTCC
5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5'
AxyI, BliHKI, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI
Bacillus subtilis 54 5' GGNCC
3' CCNGG
5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'
AsuI, Bac36I, BavBII, BsiZI, BspBII, CcuI, MaeK81II, PspPI
Bacillus subtilis 1532 5' CGCG
3' GCGC
5' ---CG   CG--- 3'
3' ---GC   GC--- 5'
BceBI, Bpu95I, Bsp123I, BstUI, BtkI, Csp68KVI, FnuDII, MvnI, ThaI
Bacillus subtilis 1854 5' GRGCYC
3' CYCGRG
5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
Bacillus subtilis 5' CTGCAG
3' GACGTC
5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5'
AjoI, BloHII, CflI, CstI, Ecl37kI, HalII, Psp23I, PstI, SalPI, SflI, YenI
Bacillus subtilis 5' CCGG
3' GGCC
5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
Bacillus subtilis 168 5' CTCGAG
3' GAGCTC
5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5'
XhoI, BssHI, TliI, PaeR7I, Sfr274I, SlaI, StrI
Bacillus subtilis R 5' GGCC
3' CCGG
5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
HaeIII
Bacillus subtilis TU 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
AagI, BanIII, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, ClaI
5' GGCC
3' CCGG
5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
Bacillus thermoglucosidasius 5' CCRYGG
3' GGYRCC
5' ---C   CRYGG--- 3'
3' ---GGYRC   C--- 5'
Bacillus thermoglucosidasius 5' GCGATG
3' CGCTAC
5' ---GCGATGN8NN   NNNN--- 3'
3' ---CGCTACN8NNNNNN   --- 5'
Bacillus thuringiensis AII 5' GGWCC
3' CCWGG
5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5'
BamNxI, BcuAI, BsrAI, Eco47I, FspMSI, HgiCII, HgiJI, SmuEI
Bacillus thuringiensis 2294 5' GCNGC
3' CGNCG
5' ---GCNG   C--- 3'
3' ---C   GNCG--- 5'
Bacillus thuringiensis D4 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AorI, ApyI, Bse24I, BstNI, BstOI, Bst2UI, CbrI, EcoRII, MvaI, SleI
Bacillus thuringiensis E 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaI, SleI
Bacillus thuringiensis Kumantoensis 5' CGCG
3' GCGC
5' ---CG   CG--- 3'
3' ---GC   GC--- 5'
BepI, Bpu95I, Bsp123I, BstFNI, BstUI, BtkI, FalII, SelI, ThaI
Bacillus thuringiensis Kumantoensis 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
BfuCI, Bsp67I, Bst19II, CcyI, FnuCI, MboI, NmeCI, SauMI
Bacillus stearothermophilus SE-U62 5' CACGTC
3' GTGCAG
5' ---CAC   GTC--- 3'
3' ---GTG   CAG--- 5'
Bacillus thermoglucosidasius 5' GCAGTG
3' CGTCAC
5' ---GCAGTGNN   --- 3'
3' ---CGTCAC   NN--- 5'
5' ACCTGC
3' TGGACG
5' ---ACCTGCNNNN   NNNN--- 3'
3' ---TGGACGNNNNNNNN   --- 5'
Acc36I, BfuAI, BspMI
5' GRGCYC
3' CYCGRG
5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
Bacteroides vulgatus 5' CGTACG
3' GCATGC
5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'

Notes[]

  1. ^ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
  2. ^ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
  3. ^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
  4. ^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
  5. ^ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
  6. ^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
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