List of restriction enzyme cutting sites: A

From Wikipedia, the free encyclopedia
Main articles: List of restriction enzyme cutting sites and Restriction enzyme

Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

This article contains a list of restriction enzymes whose names start with A and have a clearly defined cutting site.

The following information is given for each enzyme:

  • Name of Restriction Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature,[1][2] and bibliographical references. Note: When alphabetizing, enzymes are first ordered alphabetically by the acronyms (everything before the roman numeral); then enzymes of a given acronym are ordered alphabetically by the roman numeral, treating the numeral as a number and not a string of letters. This helps keep the entries ordered hierarchically while also alphabetic.(Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action.[3][4]
  • REBASE Number: Number used to identify restriction enzymes in the REBASE restriction enzyme database. This database includes important information about the enzyme such as Recognition sequence, source, and Isoschizomers, as well as other data, such as the commercial suppliers of the enzyme.
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Displays the cut site and pattern and products of the cut. The recognition sequence and the cut site usually match, but sometimes the cut site can be dozens of nucleotides away from the recognition site.[5][6]
  • Isoschizomers and neoschizomers: An isoschizomer is a restriction enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8–10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None as of [date]" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists, as in this not listed enzyme:  Abc123I 

Whole list navigation[]

Restriction enzymes[]

A[]

Enzyme PDB code REBASE Number Source Recognition sequence Cut Isoschizomers
AaaI 1 Acetobacter aceti 5' CGGCCG
3' GCCGGC 		5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5' 		BseX3I, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII
4 Achromobacter agile 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BanIII, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI
15358 Arthrobacter aurescens RFL2 5' TTATAA
3' AATATT 		5' ---TTA   TAA--- 3'
3' ---AAT   ATT--- 5' 		PsiI, Csp1BORF7380P
[7] 2892 Arthrobacter aurescens SS2-322 5' CACCTGC
3' GTGGACG 		5' ---CACCTGCN3N   NNNN--- 3'
3' ---GTGGACGN3NNNNN   --- 5' 		None as of May 2010
5465 Arthrobacter aurescens RFL3 5' GACN6GTC
3' CTGN6CAG 		5' ---GACNNNN   NNGTC--- 3'
3' ---CTGNN   NNNNCAG--- 5' 		DrdI, DseDI
[8] 6 Acetobacter aceti 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AspMI, Eco147I,  Eco1524I,  PceI, Pme55I, SarI, Sru30DI, SteI
[8][9][10][11] 7 Acetobacter aceti 5' GACGTC
3' CTGCAG 		5' ---GACGT   C--- 3'
3' ---C   TGCAG--- 5' 		Ssp5230I, ZraI
3010 Arthrobacter aurescens 5' TGTACA
3' ACATGT 		5' ---T   GTACA--- 3'
3' ---ACATG   T--- 5' 		 BsmRI,  Bsp1407I, BsrGI, BstAUI, Ssp4800I, SspBI
3029 Azospirillum brasilense UQ 1796 5' TGATCA
3' ACTAGT 		5' ---T   GATCA--- 3'
3' ---ACTAG   T--- 5' 		BclI, BsiQI, BspXII, BstT7I, FbaI, Ksp22I,  ParI 
3097 5' CCTCAGC
3' GGAGTCG 		5' ---CC   TCAGC--- 3'
3' ---GGAGT   CG--- 5' 		BbvCI
8 Azospirillum brasilense 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BluI, BspAAI, BssHI, EscI, PaeR7I, SciI, Sfr274I, Sol10179I, StrI, TliI
14594 Arthrobacter sp. 7M06 5' CCTCGAGG
3' GGAGCTCC 		5' ---CC   TCGAGG--- 3'
3' ---GGAGCT   CC--- 5' 		None as of 4 Apr. 2019
[12][13][14][15] 18 Acinetobacter calcoaceticus 5' GTMKAC
3' CAKMTG 		5' ---GT   MKAC--- 3'
3' ---CAKM   TG--- 5' 		FblI, XmiI
[12][16] 19 Acinetobacter calcoaceticus 5' CGCG
3' GCGC 		5' ---CG   CG--- 3'
3' ---GC   GC--- 5' 		Bsh1236I, Bsp50I, BstFNI, BstUI, MvnI, SelI, BceBI, BepI, Bpu95I
[17] 20 Acinetobacter calcoaceticus 5' TCCGGA
3' AGGCCT 		5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5' 		Aor13HI, BbvAIII, BlfI, BseAI, BsiMI, BspEI, CauB3I, Kpn2I, MroI, PtaI
2638 Acinetobacter calcoaceticus 16 5' TGCGCA
3' ACGCGT 		5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5' 		AosI, AviII, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
[18] 4162 Acinetobacter calcoaceticus 36 5' ACCTGC
3' TGGACG 		5' ---ACCTGCN3N   NNNN--- 3'
3' ---TGGACGN3NNNNN   --- 5' 		BfuAI, BspMI, BveI
[19] 14 Acinetobacter calcoaceticus 65 5' GGTACC
3' CCATGG 		5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5' 		AhaB8I, Asp718I, KpnI, SthI
2665 Acinetobacter calcoaceticus 113 5' AGTACT
3' TCATGA 		5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5' 		AssI,  BmcAI, Bpa34I,  DpaI, Eco255I, RflFII, ScaI, ZrmI
15 Acinetobacter calcoaceticus B1 5' GGYRCC
3' CCRYGG 		5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5' 		BanI, BbvBI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I
2666 Acinetobacter calcoaceticus B2 5' RGCGCY
3' YCGCGR 		5' ---RGCGC   Y--- 3'
3' ---Y   CGCGR--- 5' 		 BfoI,  Bme142I, Bsp143II, BstH2I, HaeII, LpnI
[20] 16 Acinetobacter calcoaceticus B7 5' CCAN5TGG
3' GGTN5ACC 		5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5' 		AcpII, Asp10HII, BasI, Esp1396I, PflBI, PflMI, Van91I
[21] 2733 Acinetobacter calcoaceticus BS 5' CCGCTC
3' GGCGAG 		5' ---CCG   CTC--- 3'
3' ---GGC   GAG--- 5' 		BsrBI, BstD102I, Bst31NI, MbiI
17 Acinetobacter calcoaceticus EBF 65/65 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AsiI, BamHI, Bce751I, Bsp98I, BspAAIII, CelI, OkrAI, Uba4009I
2571 5' GCWGC
3' CGWCG 		5' ---G   CWGC--- 3'
3' ---CGWC   G--- 5' 		ApeKI, SuiI, Taq52I, TseI
2570 5' GCTAGC
3' CGATCG 		5' ---GCTAG   C--- 3'
3' ---C   GATCG--- 5' 		AsuNHI, BmtI,  BspOI, , LlaG2I, NheI, PstNHI
2569 5' CAGCTC
3' GTCGAG 		5' ---CAGCTCN5N   NNNN--- 3'
3' ---GTCGAGN5NNNNN   --- 5' 		— None as of May 2010 —
[11][22] 21 Arthrobacter citreus 5' CCGC
3' GGCG 		5' ---C   CGC--- 3'
3' ---GGC   G--- 5' 		SsiI
[23] 2181 Acinetobacter calcoaceticus M4 5' AACGTT
3' TTGCAA 		5' ---AA   CGTT--- 3'
3' ---TTGC   AA--- 5' 		Psp1406I
2667 Acinetobacter calcoaceticus N20 5' ACTAGT
3' TGATCA 		5' ---A   CTAGT--- 3'
3' ---TGATC   A--- 5' 		AhlI, BcuI, SpeI
2668 Acinetobacter calcoaceticus W2131 5' GGATC
3' CCTAG 		5' ---GGATCN3N   N--- 3'
3' ---CCTAGN3NN   --- 5' 		AlwI, BinI,  BpuFI, BsrWI,  BspPI, BstH9I,  Bst31TI,  EacI,  Ral8I 
11822 Acinetobacter calcoaceticus 5' YGGCCR
3' RCCGGY 		5' ---Y   GGCCR--- 3'
3' ---RCCGG   Y--- 5' 		EaeI, Bfi89I, CfrI, EcoHK31I
22 Acidiphilium cryptum 25H 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		Asp10HI, Bpu14I, Bsp119I, BstBI, Csp45I, FspII, MlaI, NspV, SspRFI
23 Acidiphilium cryptum 25H 5' CCAN5TGG
3' GGTN5ACC 		5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5' 		AccB7I, Asp10HII, BasI, Esp1396I, PflBI, PflMI, Van91I
25 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AspAI, Bse64I, BseT9I, BstEII, BstPI, Eco91I, EcoO65I, PspEI
[24] 2184 Arthrobacter citreus 310 5' RAATTY
3' YTTAAR 		5' ---R   AATTY--- 3'
3' ---YTTAA   R--- 5' 		ApoI,  CfaI,  FsiI, XapI
5534 Acinetobacter calcoaceticus 5' CTGAAG
3' GACTTC 		5' ---CTGAAGN13NNN   --- 3'
3' ---GACTTCN13N   NN--- 5' 		BspKT5I, Eco57I
5359 Aeromonas caviae 5' CACGTG
3' GTGCAC 		5' ---CAC   GTG--- 3'
3' ---GTG   CAC--- 5' 		BbrPI, BcoAI, Eco72I, PmaCI, PmlI, PspCI
[25] 31 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AhaII, AosII, BbiII, BsaHI, BstACI, Hin1I, HgiI, Hsp92I, Msp17I
2991 Alcaligenes denitrificans Ss 3-028 5' CACNNNGTG
3' GTGNNNCAC 		5' ---CACNNN   GTG--- 3'
3' ---GTG   NNNCAC--- 5' 		BstIZ316I, DraIII
33 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AglI, AjnI, BseBI, BstNI, BstOI, Bst2UI, EcoRII, SleI, SspAI
[26] 37 5' GTAC
3' CATG 		5' ---GT   AC--- 3'
3' ---CA   TG--- 5' 		Csp6I, CviQI, CviRII, HpyBI, PabI, PlaAII, RsaI, RsaNI
34 Acidiphilium sp. 16R 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa22MI, BspCI, EagBI, MvrI, NblI, Ple19I, PvuI, Psu161I, RshI, XorII
35 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa16RI, BspCI, EagBI, ErhB9I, NblI, Ple19I, Psu161I, PvuI, RshI
[27] 2669 Alcaligenes faecalis T2774 5' AGCGCT
3' TCGCGA 		5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5' 		AitI, Aor51H, Eco47III, FunI
10489 5' CCN7GG
3' GGN7CC 		5' ---CCNNNNN   NNGG--- 3'
3' ---GGNN   NNNNNCC--- 5' 		Bsc4I, BseLI, BslI, BflI, Bsc107I, BsiYI, Bst22I
[28] Anabaena flos-aquae 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Asp745I, BamNI, BcuAI, CauI, Csp68KI, DsaIV, Eco47I, SinI
[28][29][30] 39 Anabaena flos-aquae 5' CTTAAG
3' GAATTC 		5' ---C   TTAAG--- 3'
3' ---GAATT   C--- 5' 		BfrI, BspTI, Bst98I, BstAFI, BstPZ740I, Esp4I, MspCI, Vha464I
[28][10] 40 Anabaena flos-aquae 5' ACRYGT
3' TGYRCA 		5' ---A   CRYGT--- 3'
3' ---TGYRC   A--- 5' 		 Asp90I 
[11][31] 42 5' ACCGGT
3' TGGCCA 		5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5' 		AsiAI, AsiGI, BshTI, CsiAI, CspAI, PinAI
[32] 6652 Arthrobacter globiformis 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AeuI, Bse16I, Bse17I, Bse24I, BshGI, EcoRII, SleI, SniI, SslI
Agrococcus sp. 25 5' TTSAA
3' AASTT 		5' ---TTS   AA--- 3'
5' ---AA   STT--- 3' 		None as of 4 May 2019
45 5' CCSGG
3' GGSCC 		5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5' 		AseII, BcnI,  BpuMI,  CauII, Eco1831I, EcoHI, HgiS22I, NciI
5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AstWI, AsuIII, BsaHI, BstACI, Hin1I, HgiI, HgiDI, HgiHII, PamII
[14][29][30][33] 5' TTTAAA
3' AAATTT 		5' ---TTT   AAA--- 3'
3' ---AAA   TTT--- 5' 		DraI, PauAII, SruI
5' GGTACC
3' CCATGG 		5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5' 		Acc65I, Asp718I, KpnI, SthI
[34][35] Aeromonas hydrophila 5' GACN5GTC
3' CTGN5CAG 		5' ---GACNNN   NNGTC--- 3'
3' ---CTGNN   NNNCAG--- 5' 		AspEI,  BmeRI,  BspOVI, DriI, Eam1105I, EclHKI, NruGI
4838 5' ACTAGT
3' TGATCA 		5' ---A   CTAGT--- 3'
3' ---TGATC   A--- 5' 		AclNI, BcuI, SpeI
AhyI 49 Aeromonas hydrophila 5' CCCGGG
3' GGGCCC 		5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5' 		Cfr9I, CfrJ4I, EaeAI, EclRI, PaeBI, SmaI, TspMI, XmaI, XmaCI
54 5' AGCGCT
3' TCGCGA 		5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5' 		AfeI, Aor51H, Eco47III, FunI
10919 Acinetobacter johnsonii RFL47 5' CACGTC
3' GTGCAG 		5' ---CAC   GTC--- 3'
3' ---GTG   CAG--- 5' 		BmgBI, BtrI
7185 Acinetobacter johnsonii R2 5' CCWGG
3' GGWCC 		5' ---   CCWGG--- 3'
3' ---GGWCC   --- 5' 		AorI, ApaORI, ApyI, EcoRII, MvaI, Psp6I, PspGI, SleI, SspAI
2604 Acinetobacter johnsonii 35 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		ApiI, BloHII, BspMAI, CfrA4I, CfuII, PstI, SalPI, SflI, Srl5DI
7740 Acinetobacter junii RFL46 5' GAAN7TTGG
3'CTTN7AACC 		5' ---NNNNN   NN6GAAN7TTGGN5NNNNNN   --- 3'
3' ---   NNNNNNN6CTTN7AACCN5N   NNNNN--- 5' 		None as of 28 Oct. 2020
5791 5' CACN4GTG
3' GTGN4CAC 		5' ---CACNN   NNGTG--- 3'
3' ---GTGNN   NNCAC--- 5' 		OliI
6110 Acinetobacter lwoffii BH 32 5' GCAN6TGC
3' CGTN6ACG 		5' ---GCAN6TGCN9NNN   --- 3'
3' ---CGTN6ACGN9N   NN--- 5' 		None as of May 2010
5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AccEBI, BamHI, Bce751I, Bsp98I, BspAAIII, Nsp29132II, Pfl8I
59 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		ApiI, Asp713I, BspMAI, CflI, CstI, MhaAI, PstI, SalPI, Sst12I, YenI
[36][37] 2812 Acinetobacter lwoffii Ks 4-8 5' GAACN6TCC
3' CTTGN6AGG 		5' ---GAACN6TCCN6NNNNNN   --- 3'
3' ---CTTGN6AGGN6N   NNNNN--- 5' 		None as of May 2010
[12][14][38][39][40][41] 61 Arthrobacter luteus 5' AGCT
3' TCGA 		5' ---AG   CT--- 3'
3' ---TC   GA--- 5' 		AluBI, MltI
16189 Arthrobacter luteus B 5' AGCT
3' TCGA 		5' ---AG   CT--- 3'
3' ---TC   GA--- 5' 		AluI, MltI
65 Acinetobacter lwoffii 5' GGATC
3' CCTAG 		5' ---GGATCN3N   N--- 3'
3' ---CCTAGN3NN   --- 5' 		AclWI, BinI, BspPI,  BsrWI,  BstH9I,  BthII, Bth617I,  EacI
Acinetobacter lwoffii RFL21 5' GWGCWC
3' CWCGWG 		5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5' 		AspHI, Bbv12I, Bsh45I, BsiHKAI, Bsm6I,  HgiAI, MspV281I
[42] 63 Acinetobacter lwoffii RFL26 5' GTCTC
3' CAGAG 		5' ---GTCTCN   NNNN--- 3'
3' ---CAGAGNNNNN   --- 5' 		 BcoDI, BscQII,   BsmAI, BsoMAI,  BstMAI 
Acinetobacter lwoffii RFL44 5' GTGCAC
3' CACGTG 		5' ---G   TGCAC--- 3'
3' ---CACGT   G--- 5' 		ApaLI, SnoI, VneI
[43] Acinetobacter lwoffii N 5' CAGNNNCTG
3' GTCNNNGAC 		5' ---CAGNNN   CTG--- 3'
3' ---GTC   NNNGAC--- 5' 		CaiI
67 Acinetobacter lwoffii X 5' GCAGC
3' CGTCG 		5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5' 		BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I
Alteromonas macleodii 87 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		AquI, AvaI,  BmeT110I,  BsiHKCI, BsoBI, Eco88I, Nli3877I, NspSAI
26714 5' GCTCCA
3' CGAGGT 		5' ---GCTCCAN8NNN   --- 3'
3' ---CGAGGTN8N   NN--- 5' 		None as of 4 May 2018
74 Anabaena sp. 5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AyI, Bse21I, Bsu36I, Eco81I, Lmu60I, OaNI, MstII, SauI, SshAI
Anabaena sp. 5' GDGCHC
3' CHCGDG 		5' ---GDGCH   C--- 3'
3' ---C   HCGDG--- 5' 		BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII, SduI
77 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		BseBI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, Sth117I, Tai
[44] 5' TCCGGA
3' AGGCCT 		5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5' 		AccIII, BbvAIII, BlfI, BseAI, BsiMI, BspEI, CauB3I, Kpn2I, MroI, PtaI
[45] 76 5' AGCGCT
3' TCGCGA 		5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5' 		AfeI, AitI, Eco47III, FunI
5' TGCGCA
3' ACGCGT 		5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5' 		Acc16I, AviII, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AstWI, BbiII, BsaHI, BstACI, Hin1I, HgiI, HgiGI, Msp17I, PamII
[11][30][46] 5' GGGCCC
3' CCCGGG 		5' ---GGGCC   C--- 3'
3' ---C   CCGGG--- 5' 		Bsp120I, PpeI, PspOMI
[47] 5' GCAN5TGC
3' CGTN5ACG 		5' ---GCANNNNN   TGC--- 3'
3' ---CGT   NNNNNACG--- 5' 		BstAPI
5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AliI, BamHI, BnaI, Bsp4009I, BstI, NspSAIV, Pfl8I, SolI, SurI
[48][49] 5' GTGCAC
3' CACGTG 		5' ---G   TGCAC--- 3'
3' ---CACGT   G--- 5' 		Alw44I, SnoI, VneI
86 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AjnI, BstNI, BstOI, Bst1I, Bst2I, Fsp1604I, MvaI, Psp6I, PspGI
[50] Aeropyrum pernix K1 5' GCWGC
3' CGWCG 		5' ---G   CWGC--- 3'
3' ---CGWC   G--- 5' 		AceI, SuiI, Taq52I, TseI
2315 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		Asp713I, Bsp63I, CfrA4I, PaePI, Pfl21I, Psp23I, PstI, SflI, Sst12I
[51] Arthrobacter protophormiae 5' RAATTY
3' YTTAAR 		5' ---R   AATTY--- 3'
3' ---YTTAA   R--- 5' 		AcsI, CfaI,  FsiI, XapI, EcoRI
25962 Arthrospira platensis NIES-39 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		See this site for a complete list.
93 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		BciBII, BptI, BseBI, BstNI, BstOI, Bst2UI, Bst38I, Bst100I, MvaI
14756 Arcanobacterium pyogenes 5' ATCGAC
3' TAGCTG 		5' ---ATCGACN17NNN   --- 3'
3' ---TAGCTGN17N   NN--- 5' 		None as of 4 August 2019
5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		BcoI,  BmeT110I,  BsmAI, BsoMAI, BsoBI, BstSI, Eco27kI, OfoI
20238 5' GCCGNAC
3' CGGCNTG 		5' ---GCCGNACN17NNN   --- 3'
3' ---CGGCNTGN17N   NN--- 5' 		None as of 4 August 2019
20239 5' GAGGAG
3' CTCCTC 		5' ---GAGGAGN17NNN   --- 3'
3' ---CTCCTCN17N   NN--- 5' 		BseRI
20240 5' GRGGAAG
3' CYCCTTC 		5' ---GRGGAAGN16NNN   --- 3'
3' ---CYCCTTCN16N   NN--- 5' 		None as of 4/9/19
19405 Arthrobacter sp. NTS 5' GACN6TTYG
3' CTGN6AARC 		5' ---NNNNN   NN7GACN6TTYGN5NNNNNN   --- 3'
3' ---   NNNNNNN7CTGN6AARCN5N   NNNNN--- 5' 		None as of Oct. 28 2020
[11][52] Arthrobacter sp. 5' GGCGCGCC
3' CCGCGCGG 		5' ---GG   CGCGCC--- 3'
3' ---CCGCGC   GG--- 5' 		 PalAI, SgsI 
[53] 96 Aquaspirillum serpens 5' ATTAAT
3' TAATTA 		5' ---AT   TAAT--- 3'
3' ---TAAT   TA--- 5' 		AsnI, BpoAI, PshBI, Sru4DI, VspI
[53] 97 Aquaspirillum serpens 5' CCSGG
3' GGSCC 		5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5' 		AhaI, AsuC2I, BcnI, CauII, EcoHI, HgiS22I, Kpn49kII, Mgl14481I, NciI
Azotobacter sp. 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		ApaCI, BamHI, BnaI, Bsp4009I, BstI, OkrAI, Pfl8I, SurI, Uba4009I
2853 Arthrobacter sp. A7359 5' ACCGGT
3' TGGCCA 		5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5' 		AgeI, AsiGI, BshTI, CsiAI, CspAI, PinAI
10828 Arthrobacter sp. G 5' ACCGGT
3' TGGCCA 		5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5' 		AgeI, AsiAI, BshTI, CsiAI, CspAI, PinAI
Arthrobacter sp. 5' GCGATCGC
3' CGCTAGCG 		5' ---GCGAT   CGC--- 3'
3' ---CGC   TAGCG--- 5' 		 RgaI, SfaAI, , SgfI
18472 Arthrobacter sp. Ck256 5' GATC
3' CTAG 		5' ---G   ATC--- 3'
3' ---CTA   G--- 5' 		See this site for a complete list.
15803 Arthrobacter sp. Mn372 5' ATGCAT
3' TACGTA 		5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5' 		Ppu10I, EcoT22I, Mph1103I, NsiI, Zsp2I, BfrBI, Csp68KIII, PinBI, SepI, SspD5II
[54] 98 Arthrobacter sp. N-CM 5' ATTAAT
3' TAATTA 		5' ---AT   TAAT--- 3'
3' ---TAAT   TA--- 5' 		AseI, BpoAI, PshBI, Sru4DI, VspI
[55] Achromobacter sp. 699 5' GACNNNGTC
3' CTGNNNCAG 		5' ---GACN   NNGTC--- 3'
3' ---CTGNN   NCAG--- 5' 		AtsI, PflFI, PsyI, TelI, Tth111I
Acidiphilium sp. 10H 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, BspT104I, BstBI, CbiI, Csp68KII, PlaII, PpaAI, Ssp1I
100 Acidiphilium sp. 10H 5' CCAN5TGG
3' GGTN5ACC 		5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5' 		AccB7I, AcpII, BasI, Esp1396I, PflBI, PflMI, Van91I
111 Acidiphilium sp. 26H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, BsmI, PctI
112 Acidiphilium sp. 27H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp35HI, Asp36HI, Asp40HI,  BmaHI,  BsaMI, BscCI, BsmI
117 Acidiphilium sp. 35H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp36HI, Asp40HI, Asp50HI, BsaMI, BscCI, BsmI, Mva1269I
118 Acidiphilium sp. 36H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp40HI, Asp50HI,  BmaHI,  BscCI, BsmI, Mva1269I, PctI
120 Acidiphilium sp. 40H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp27HI, Asp36HI, Asp50HI, BsaMI, BscCI, BsmI, Mva1269I
122 Acidiphilium sp. 50H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp26HI, Asp35HI, Asp36HI, BsaMI, BscCI, BsmI, Mva1269I
[56] Achromobacter sp. 700 5' GAAN4TTC
3' CTTN4AAG 		5' ---GAANN   NNTTC--- 3'
3' ---CTTNN   NNAAG--- 5' 		BbvAI, MroXI, PdmI, XmnI
131 Achromobacter sp. 713 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		AjoI, AliAJI, BloHII, CfrA4I, Ecl37kI, MhaAI, PstI, SflI, Srl5DI, YenI
[57] Achromobacter sp. 718 5' GGTACC
3' CCATGG 		5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5' 		Acc65I, AhaB8I, KpnI, SthI
Achromobacter sp. 745 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, BsrAI, CauI, Eco47I, ErpI, FdiI, SinI, VpaK11AI, VpaK11BI
Alcaligenes sp. 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AcrII, Bse64I, BseT10I, BstEII, BstPI, Eco91I, EcoO65I, PspEI
6959 Arthrobacter sp. A2 5' CCTAGG
3' GGATCC 		5' ---C   CTAGG--- 3'
3' ---GGATC   C--- 5' 		AvrBII, AvrII, BlnI, BspA2I, XmaJI
[58] 5' GACN5GTC
3' CTGN5CAG 		5' ---GACNNN   NNGTC--- 3'
3' ---CTGNN   NNNCAG--- 5' 		AhdI,  BmeRI,  BspOVI, DriI, Eam1105I, EclHKI, NruGI
[59] Achromobacter sp. H 5' GWGCWC
3' CWCGWG 		5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5' 		Alw21I, Bbv12I, Bsh45I, BsiHKAI,  Bsm6I,  HgiAI, MspV281I
Arthrobacter sp. LE3860 5' GCGC
3' CGCG 		5' ---GCG   C--- 3'
3' ---C   GCG--- 5' 		BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI
2977 Acinetobacter sp. M 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI, StuI
Alcaligenes sp. MD1 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BfuCI, Bsp143I, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
Anabaena sp. J3 5' GGNNCC
3' CCNNGG 		5' ---GGN   NCC--- 3'
3' ---CCN   NGG--- 5' 		 BmiI,  BscBI, BspLI, NlaIV, PspN4I
Arthrobacter sp. S9 5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AsuI, AvcI, Bac36I, Bal228I, FmuI, NspIV, PspPI, Sau96I
4930 Arthrobacter sp. 5' AGTACT
3' TCATGA 		5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5' 		Acc113I,  BmcAI, Bpa34I,  DpaI, Eco255I, RflFII, ScaI, ZrmI
Anabaena sp. 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AsuIII, BsaHI, BstACI, Hin1I, HgiI, HgiDI, HgiGI, Msp17I
[60] 5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AspS9I, AvcI, CcuI, Cfr13I, FmuI, MaeK81II, Nsp7121I, PspPI, UnbI
[61] 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AcpI, Bpu14I, Bsp119I, BspT104I, Csp45I, FspII, LspI, NspV, SfuI
5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AsuIII, BbiII, BsaHI, BstACI, Hin1I, HgiHII, Hsp92I, Msp17I
2901 Actinobacillus suis C2 5' CCSGG
3' GGSCC 		5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5' 		AhaI, AseII, BcnI, Eco1831I, EcoHI, Kpn49kII, Mgl14481I, NciI
[62] 2811 Actinobacillus suis HP 5' GGTGA
3' CCACT 		5' ---GGTGAN6NN   --- 3'
3' ---CCACTN6N   N--- 5' 		HphI, SspD5I
[62] Actinobacillus suis NH 5' GCTAGC
3' CGATCG 		5' ---G   CTAGC--- 3'
3' ---CGATC   G--- 5' 		AceII, BmtI,  BspOI,  LlaG2I, NheI, PstNHI
5' GACNNNGTC
3' CTGNNNCAG 		5' ---GACN   NNGTC--- 3'
3' ---CTGNN   NCAG--- 5' 		AspI, PflFI, PsyI, TelI, Tth111I
[14][63][64] Anabaena variabilis 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		AquI, Ama87I, BsiHKCI, BsoBI, BspLU4I, Eco88I, NspIII, PlaAI
[12][14][41][63][64] Anabaena variabilis 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, Bme216I, BsrAI, Eco47I, Kzo49I, Psp03I, SmuEI, VpaK11AI
5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AspS9I, BshKI, BsiZI, Bsp1894I, BspBII, BspF4I, Bsu54I, Pde12I
Anabaena variabillis (halle) 5' TGCGCA
3' ACGCGT 		5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5' 		Acc16I, AosI, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
[11][29] 173 Anabaena variabilis UW 5' CCTAGG
3' GGATCC 		5' ---C   CTAGG--- 3'
3' ---GGATC   C--- 5' 		AspA2I, AvrBII, BlnI, BspA2I, XmaJI
171 5' CCTAGG
3' GGATCC 		5' ---C   CTAGG--- 3'
3' ---GGATC   C--- 5' 		AspA2I, AvrII, BlnI, BspA2I, XmaJI
[65] 174 5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AocI, BliHKI, Bse21I, BspR7I, Bsu36I, CvnI, Eco81I, Lmu60I

§ An HF version of this enzyme is available

Notes[]

  1. ^ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
  2. ^ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
  3. ^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
  4. ^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. Bibcode:1973Sci...181..223A. doi:10.1126/science.181.4096.223. PMID 4124164.
  5. ^ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
  6. ^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
  7. ^ Grigaite R, Maneliene Z, Janulaitis A (November 2002). "AarI, a restriction endonuclease from Arthrobacter aurescens SS2-322, which recognizes the novel non-palindromic sequence 5'-CACCTGC(N)4/8-3'". Nucleic Acids Res. 30 (21): 123e–123. doi:10.1093/nar/gnf122. PMC 135850. PMID 12409482.
  8. ^ a b Sugisaki H, Maekawa Y, Kanazawa S, Takanami M (October 1982). "New restriction endonucleases from Acetobacter aceti and Bacillus aneurinolyticus". Nucleic Acids Res. 10 (19): 5747–52. doi:10.1093/nar/10.19.5747. PMC 320926. PMID 6292849.
  9. ^ Nwankwo DO, Maunus RE, Xu S (January 1997). "Cloning and expression of AatII restriction-modification system in Escherichia coli". Gene. 185 (1): 105–9. doi:10.1016/S0378-1119(96)00641-5. PMID 9034320.
  10. ^ a b Roberts RJ (January 1983). "Restriction and modification enzymes and their recognition sequences". Nucleic Acids Res. 11 (1): r135–69. PMC 325705. PMID 6306557.
  11. ^ a b c d e f Wei H, Therrien C, Blanchard A, Guan S, Zhu Z (May 2008). "The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases". Nucleic Acids Res. 36 (9): e50. doi:10.1093/nar/gkn182. PMC 2396408. PMID 18413342. S2CID 10463398.
  12. ^ a b c d Nishigaki K, Kaneko Y, Wakuda H, Husimi Y, Tanaka T (August 1985). "Type II restriction endonucleases cleave single-stranded DNAs in general". Nucleic Acids Res. 13 (16): 5747–60. doi:10.1093/nar/13.16.5747. PMC 321909. PMID 2994012.
  13. ^ Shenoy S, Daigle K, Ehrlich KC, Gehrke CW, Ehrlich M (June 1986). "Hydrolysis by restriction endonucleases at their DNA recognition sequences substituted with mismatched base pairs". Nucleic Acids Res. 14 (11): 4407–20. PMC 311455. PMID 3012472.
  14. ^ a b c d e Nelson M, Christ C, Schildkraut I (July 1984). "Alteration of apparent restriction endonuclease recognition specificities by DNA methylases". Nucleic Acids Res. 12 (13): 5165–73. doi:10.1093/nar/12.13.5165. PMC 318911. PMID 6087274.
  15. ^ Nelson PS, Papas TS, Schweinfest CW (February 1993). "Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios". Nucleic Acids Res. 21 (3): 681–6. doi:10.1093/nar/21.3.681. PMC 309169. PMID 8441677.
  16. ^ Kita K, Hiraoka N, Kimizuka F, Obayashi A (1984). "Determination of the cleavage site of restriction enzyme, AccII, using synthetic oligonucleotide". Agric Biol Chem. 48 (2): 531–2. doi:10.1271/bbb1961.48.531.
  17. ^ Kita K, Hiraoka N, Oshima A, Kadonishi S, Obayashi A (December 1985). "AccIII, a new restriction endonuclease from Acinetobacter calcoaceticus". Nucleic Acids Res. 13 (24): 8685–94. doi:10.1093/nar/13.24.8685. PMC 318944. PMID 3001647.
  18. ^ Bath AJ, Milsom SE, Gormley NA, Halford SE (February 2002). "Many type IIs restriction endonucleases interact with two recognition sites before cleaving DNA". J Biol Chem. 277 (6): 4024–33. doi:10.1074/jbc.M108441200. PMID 11729187.
  19. ^ Prichodko GG, Rechnukova NI, Repin VE, Degtyarev SK (1991). "Determination of substrate specificity of restriction endonuclease Acc65I". Sib Biol J. 1: 59–60.
  20. ^ "Screening of strains producing restriction endonucleases in Lake Baikal". Izv Sib Otd Akad Nauk SSSR. 1: 35–37. 1990.
  21. ^ Abdurashitov MA, Kiseleva EV, Miakisheva TV, Dedkov VS, Shevchenko AV, Degtiarev CK (September–October 1997). "AccBSI – a novel restriction endonuclease from Acinetobacter calcoaceticus BS (Article in russian)". Prikl Biokhim Mikrobiol. 33 (5): 556–8 (Translation into english in pp. 496–8). PMID 9441298.
  22. ^ Polisson C, Morgan RD (October 1990). "AciI, a unique restriction endonuclease from Arthrobacter citreus which recognizes 5' CCGC3'". Nucleic Acids Res. 18 (19): 5911. doi:10.1093/nar/18.19.5911. PMC 332357. PMID 2170952.
  23. ^ Degtyarev SK, Abdurashitov MA, Kolyhalov AA, Rechkunova NI (July 1992). "AclI, a new restriction endonuclease from Acinetobacter calcoaceticus recognizing 5'-AA^CGTT-3'". Nucleic Acids Res. 20 (14): 3787. doi:10.1093/nar/20.14.3787. PMC 334039. PMID 1322531.
  24. ^ Degtyarev SK, Kolyhalov AA, Rechkunova NI, Abdurashitov MA (July 1992). "AcsI, a new restriction endonuclease from Arthrobacter citreus 310 recognizing 5'-Pu decreases AATTPy-3'". Nucleic Acids Res. 20 (14): 3789. doi:10.1093/nar/20.14.3789. PMC 334041. PMID 1322532.
  25. ^ de Waard A, Korsuize J, van Beveren CP, Maat J (December 1978). "A new sequence-specific endonuclease from Anabaena cylindrica". FEBS Lett. 96 (1): 106–10. doi:10.1016/0014-5793(78)81072-2. PMID 103749.
  26. ^ Dou D, Inagati K, Kita K, Ohshima A, Hiraoka N, Kishimoto N, Sugio T, Tano T (September 1989). "Restriction endonuclease AfaI from Acidiphilium facilis, a new isoschizomer of RsaI: purification and properties". Biochim Biophys Acta. 1009 (1): 83–6. doi:10.1016/0167-4781(89)90082-1. PMID 2790034.
  27. ^ Macícková-Cahová H, Hocek M (December 2009). "Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases". Nucleic Acids Res. 37 (22): 7612–22. doi:10.1093/nar/gkp845. PMC 2794189. PMID 19820117. S2CID 17925157.
  28. ^ a b c Whitehead PR, Brown NL (April 1985). "Three restriction endonucleases from Anabaena flos-aquae". J Gen Microbiol. 131 (4): 951–8. doi:10.1099/00221287-131-4-951. PMID 2985742.
  29. ^ a b c Rowland GC, Lim PP, Glass RE (July 1992). "'Stop-codon-specific' restriction endonucleases: their use in mapping and gene manipulation". Gene. 116 (1): 21–6. doi:10.1016/0378-1119(92)90624-X. PMID 1628840.
  30. ^ a b c Roberts RJ (March 1982). "Restriction and modification enzymes and their recognition sequences". Nucleic Acids Res. 10 (5): r117–44. doi:10.1093/nar/10.5.1770. PMC 320569. PMID 6280143.
  31. ^ Yamada Y, Mizuno H, Sato H, Akagawa M, Yamasato K (1989). "A new restriction endonuclease from Agrobacterium gelatinovorum, a marine agrobacterium (AgeI)". Agric Biol Chem. 53 (6): 1747–9. doi:10.1271/bbb1961.53.1747.
  32. ^ Sokolov NN (1995). "Search, isolation and study of restrictases". Vestn Ross Akad Med Nauk (in Russian) (2): 47–51. PMID 7756931.
  33. ^ Whitehead PR, Brown NL (July 1982). "AhaIII: A restriction endonuclease with a recognition sequence containing only A:T basepairs". FEBS Lett. 143 (2): 296–300. doi:10.1016/0014-5793(82)80120-8. PMID 6288466.
  34. ^ Marks P, McGeehan J, Wilson G, Errington N, Kneale G (June 2003). "Purification and characterisation of a novel DNA methyltransferase, M.AhdI". Nucleic Acids Res. 31 (11): 2803–10. doi:10.1093/nar/gkg399. PMC 156732. PMID 12771207.
  35. ^ Bogdanova E, Djordjevic M, Papapanagiotou I, Heyduk T, Kneale G, Severinov K (March 2008). "Transcription regulation of the type II restriction-modification system AhdI". Nucleic Acids Res. 36 (5): 1429–42. doi:10.1093/nar/gkm1116. PMC 2275141. PMID 18203750. S2CID 5338430.
  36. ^ Cesnaviciene E, Petrusyte M, Kazlauskiene R, Maneliene Z, Timinskas A, Lubys A, Janulaitis A (November 2001). "Characterization of AloI, a restriction-modification system of a new type". J Mol Biol. 314 (2): 205–16. doi:10.1006/jmbi.2001.5049. PMID 11718555.
  37. ^ Jurenaite-Urbanaviciene S, Serksnaite J, Kriukiene E, Giedriene J, Venclovas C, Lubys A (June 2007). "Generation of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains". Proc Natl Acad Sci USA. 104 (25): 10358–63. Bibcode:2007PNAS..10410358J. doi:10.1073/pnas.0610365104. PMC 1965518. PMID 17553965.
  38. ^ Blakesley RW, Wells RD (October 1975). "'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases". Nature. 257 (5525): 421–2. Bibcode:1975Natur.257..421B. doi:10.1038/257421a0. PMID 809717. S2CID 24619091.
  39. ^ Roberts RJ, Myers PA, Morrison A, Murray K (March 1976). "A specific endonuclease from Arthrobacter luteus". J Mol Biol. 102 (1): 157–65. doi:10.1016/0022-2836(76)90079-6. PMID 1271462.
  40. ^ Greene PJ, Heyneker HL, Bolivar F, Rodriguez RL, Betlach MC, Covarrubias AA, Backman K, Russel DJ, Tait R, Boyer HW (July 1978). "A general method for the purification of restriction enzymes". Nucleic Acids Res. 5 (7): 2373–80. doi:10.1093/nar/5.7.2373. PMC 342170. PMID 673857.
  41. ^ a b Huang LH, Farnet CM, Ehrlich KC, Ehrlich M (March 1982). "Digestion of highly modified bacteriophage DNA by restriction endonucleases". Nucleic Acids Res. 10 (5): 1579–91. doi:10.1093/nar/10.5.1579. PMC 320551. PMID 6280151.
  42. ^ Bitinaite J, Maneliene Z, Menkevicius S, Klimasauskas S, Butkus V, Janulaitis A (October 1992). "Alw26I, Eco31I and Esp3I—type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA". Nucleic Acids Res. 20 (19): 4981–5. doi:10.1093/nar/20.19.4981. PMC 334273. PMID 1408816.
  43. ^ Morgan RD, Dalton M, Stote R (September 1987). "A unique type II restriction endonuclease from Acinetobacter lwoffi N". Nucleic Acids Res. 15 (17): 7201. doi:10.1093/nar/15.17.7201. PMC 306225. PMID 2821501.
  44. ^ Inagaki K, Hikita T, Yanagidani S, Nomura Y, Kishimoto N, Tano T, Tanaka H (October 1993). "Restriction endonuclease Aor13HI from Acidiphilium organovorum 13H, a new isoschizomer of BspMII: Purification and characterization". Biosci Biotechnol Biochem. 57 (10): 1716–21. doi:10.1271/bbb.57.1716. PMID 7764267.
  45. ^ Sagawa H, Takagi M, Nomura Y, Inagaki K, Tano T, Kishimato N, Kotani H, Nakajima K (January 1992). "Isolation and identification of restriction endonuclease Aor51HI from Acidiphilium organovorum 51H". Nucleic Acids Res. 20 (2): 365. doi:10.1093/nar/20.2.365. PMC 310379. PMID 1741262.
  46. ^ Seurinck J, Van, de Voorde A, Van Montagu M (July 1983). "A new restriction endonuclease from Acetobacter pasteurianus". Nucleic Acids Res. 11 (13): 4409–15. doi:10.1093/nar/11.13.4409. PMC 326055. PMID 6306590.
  47. ^ Grones J, Turna J (March 1993). "Some properties of restriction endonuclease ApaBI from Acetobacter pasteurianus". Biochim Biophys Acta. 1162 (3): 323–5. doi:10.1016/0167-4838(93)90297-5. PMID 8457597.
  48. ^ Xu SY, Xiao JP, Ettwiller L, Holden M, Aliotta J, Poh CL, Dalton M, Robinson DP, Petronzio TR, Moran L, Ganatra M, Ware J, Slatko B, Benner J (November 1998). "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli". Mol Gen Genet. 260 (2–3): 226–31. doi:10.1007/s004380050890. PMID 9862476. S2CID 2275352.
  49. ^ Xia Y, Van Etten JL, Dobos P, Ling YY, Krell PJ (October 1993). "Adenine DNA methyltransferase M.CviRI expression accelerates apoptosis in baculovirus-infected insect cells". Virology. 196 (2): 817–24. doi:10.1006/viro.1993.1539. PMID 8372450.
  50. ^ Kawarabayasi Y, Hino Y, Horikawa H, Yamazaki S, Haikawa Y, Jin-no K, Takahashi M, Sekine M, Baba S, Ankai A, Kosugi H, Hosoyama A, Fukui S, Nagai Y, Nishijima K, Nakazawa H, Takamiya M, Masuda S, Funahashi T, Tanaka T, Kudoh Y, Yamazaki J, Kushida N, Oguchi A, Aoki K, Kubota K, Nakamura Y, Nomura N, Sako Y, Kikuchi H (April 1999). "Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1". DNA Res. 6 (2): 83–101, 145–52. doi:10.1093/dnares/6.2.83. PMID 10382966.
  51. ^ Polisson C, Robinson D (June 1992). "ApoI, a unique restriction endonuclease from Arthrobacter protophormiae which recognizes 5' RAATTY-3'". Nucleic Acids Res. 20 (11): 2888. doi:10.1093/nar/20.11.2888. PMC 336940. PMID 1614876.
  52. ^ Bilcock DT, Daniels LE, Bath AJ, Halford SE (December 1999). "Reactions of type II restriction endonucleases with 8-base pair recognition sites". J Biol Chem. 274 (51): 36379–86. doi:10.1074/jbc.274.51.36379. PMID 10593932.
  53. ^ a b Polisson C, Morgan RD (November 1988). "AseI, a restriction endonuclease from Aquaspirillum serpens which recognizes 5'AT—TAAT3'". Nucleic Acids Res. 16 (21): 10365. doi:10.1093/nar/16.21.10365. PMC 338867. PMID 3264065.
  54. ^ Rexer BU, Jarsch M, Sagmeister C, Glück B, Berger G, Kessler C (August 1988). "AsnI: a novel class II restriction endonuclease from Arthrobacter sp., strain N-CM, recognizing 5'-AT/TAAT-3'". FEBS Lett. 235 (1–2): 241–6. doi:10.1016/0014-5793(88)81271-7. PMID 2841156.
  55. ^ Bolton BJ, Schmitz GG, Jarsch M, Kessler C (June 1990). "AspI, a novel isoschizomer of Tth111I from Achromobacter species 699 recognizing 5'-GACN/NNGTC-3'". Nucleic Acids Res. 18 (11): 3422. doi:10.1093/nar/18.11.3422. PMC 330974. PMID 2162524.
  56. ^ Bolton BJ, Comer M, Kessler C (November 1989). "Asp700I, a novel isoschizomer of XmnI from Achromobacter species 700 recognizing 5'-GAANN/NNTTC-3'". Nucleic Acids Res. 17 (21): 8879. doi:10.1093/nar/17.21.8879. PMC 335071. PMID 2587236.
  57. ^ Mural RJ (November 1987). "Cleavage by the restriction endonuclease Asp718, an isoschizomer of KpnI, is sensitive to Escherichia coli Dcm methylation". Nucleic Acids Res. 15 (21): 9085. doi:10.1093/nar/15.21.9085. PMC 306431. PMID 2825125.
  58. ^ Frey B, Kaluza K, Auer J, Stratidakis I, Rina M, Bouriotis V, Schmitz G (July 1992). "AspEI, a novel Eam11051 isoschizomer from Aureobacterium species recognizing 5'-GACnnn/nnGTC-3'". Nucleic Acids Res. 20 (14): 3782. doi:10.1093/nar/20.14.3782. PMC 334034. PMID 1641345.
  59. ^ Bolton BJ, Höltke HJ, Schmitz GG, Jarsch M, Kessler C (November 1989). "AspHI, a novel isoschizomer of HgiAI from Achromobacter species H recognizing 5'-GWGCW/C-3'". Nucleic Acids Res. 17 (22): 9500. doi:10.1093/nar/17.22.9500. PMC 335177. PMID 2587281.
  60. ^ Hughes SG, Bruce T, Murray K (January 1980). "The Isolation and Characterization of a Sequence-Specific Endonuclease from Anabaena subcylindrica". Biochem J. 185 (1): 59–63. doi:10.1042/bj1850059. PMC 1161269. PMID 6246880.
  61. ^ Roberts RJ (March 1980). "Restriction and modification enzymes and their recognition sequences". Gene. 8 (4): 329–43. doi:10.1016/0378-1119(80)90040-2. PMID 6245015.
  62. ^ a b Dedkov VS, Degtyarev SK (April–May 1998). "Actinobacillus and Streptococcus: producers of isoschizomers of the restriction endonucleases R.HphI, R.SauI, R.NheI, R.MboI and R.SwaI". Biol Chem. 379 (4–5): 573–4. PMID 9628357.
  63. ^ a b Kaneko T, Nakamura Y, Wolk CP, Kuritz T, Sasamoto S, Watanabe A, Iriguchi M, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakazaki N, Shimpo S, Sugimoto M, Takazawa M, Yamada M, Yasuda M, Tabata S (October 2001). "Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120". DNA Res. 8 (5): 227–53. doi:10.1093/dnares/8.5.205. PMID 11759840.
  64. ^ a b Hughes SG, Murray K (January 1980). "The nucleotide sequences recognized by endonucleases AvaI and AvaII from Anabaena variabilis". Biochem J. 185 (1): 65–75. doi:10.1042/bj1850065. PMC 1161270. PMID 6246881.
  65. ^ Yoshioka H, Nakamura H, Sasaki J, Tahara Y, Yamada Y (1983). "Purification, properties and recognition sequence of site-specific restriction endonuclease from "Acetobacter xylinus"". Agric Biol Chem. 47 (12): 2871–9. doi:10.1271/bbb1961.47.2871.
Retrieved from ""