List of restriction enzyme cutting sites: S

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Main articles: List of restriction enzyme cutting sites and Restriction enzyme
Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

This article contains a list of the most studied restriction enzymes whose names start with S. It contains approximately 130 enzymes. The following information is given:

  • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
  • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme:  EcoR70I 


Whole list navigation[]

Restriction enzymes[]

S[]

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
SacI[7] Streptomyces achromogenes 5' GAGCTC
3' CTCGAG 		5' ---GAGCT   C--- 3'
3' ---C   TCGAG--- 5'
Streptomyces achromogenes 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5' 		Cfr42I
Streptomyces achromogenes N-J-H 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
[7] Streptomyces albus 5' GTCGAC
3' CAGCTG 		5' ---G   TCGAC--- 3'
3' ---CAGCT   G--- 5'
Streptomyces albus 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		ApiI, BspMAI, CstI, Ecl37kI, Ecl2zI, Psp23I, PstI, Srl5DI, YenI
Streptomyces sp. 5' GGGWCCC
3' CCCWGGG 		5' ---GG   GWCCC--- 3'
3' ---CCCWG   GG--- 5'
Saccharopolyspora sp. 5' GCTCTTC
3' CGAGAAG 		5' ---GCTCTTCN   NNN--- 3'
3' ---CGAGAAGNNNN   --- 5'
5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, Sru30DI, SseBI, SteI, StuI
Staphylococcus arlettae RFL832 5' GCNGC
3' CGNCG 		5' ---GC   NGC--- 3'
3' ---CGN   CG--- 5'
Streptomyces aureofaciens IKA 18/4 5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI
Staphylococcus aureus PS96 5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbI
Streptomyces aureofaciens 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI
2REU Staphylococcus aureus 3A 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		Bce243I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I
Streptomyces aureofaciens BM-K 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
Streptomyces aureofaciens R8/26 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
Streptomyces aureofaciens B-96 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
Streptomyces aureofaciens B-96 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI
Staphylococcus aureus M 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BscFI, BspAI, BstKTI, ChaI, HacI, MkrAI, RalF40I, SsiAI
Streptomyces aureofaciens NMU 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
Streptomyces aureofaciens 16 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
Streptomyces sp. BF-61 5' CCTGCAGG
3' GGACGTCC 		5' ---CCTGCA   GG--- 3'
3' ---GG   ACGTCC--- 5'
Streptomyces bikiniensis JAM68 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI
Shigella boydii 13 5' TCGCGA
3' AGCGCT 		5' ---TCG   CGA--- 3'
3' ---AGC   GCT--- 5'
Streptococcus bovis II/1 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
[7] Streptomyces caespitosus 5' AGTACT
3' TCATGA 		5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5'
Acc113I, AssI,  BmcAI, Bpa34I, 
DpaI, Eco255I, RflFII, ZrmI
Saccharomyces cerevisiae 5' GCCGGC
3' CGGCCG 		5' ---G   CCGGC--- 3'
3' ---CGGCC   G--- 5'
Staphylococcus cohnii Lki 19-320 5' GAGTC
3' CTCAG 		5' ---GAGTCN4N   --- 3'
3' ---CTCAGN4N   --- 5'
5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Streptoverticillium cinnamoneum 5' CTCGAG
3' GAGCTC 		5' ---CTC   GAG--- 3'
3' ---GAG   CTC--- 5' 		BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SlaI, Sol10179I, XhoI
Spiroplasma citri ASP2 5' GCGC
3' CGCG 		5' ---G   CGC--- 3'
3' ---CGC   G--- 5' 		BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI
Streptococcus cremoris F 5' CCNGG
3' GGNCC 		5' ---CC   NGG--- 3'
3' ---GGN   CC--- 5'
2IXS Streptomyces diastaticus Ng7-324 5' CCTGCAGG
3' GGACGTCC 		5' ---CCTGCA   GG--- 3'
3' ---GG   ACGTCC--- 5'
Streptomyces diastaticus 5' GGCCN5GGCC
3' CCGGN5CCGG 		5' ---GGCCNNNN   NGGCC--- 3'
3' ---CCGGN   NNNNCCGG--- 5'
Streptococcus durans RFL3 5' GDGCHC
3' CHCGDG 		5' ---GDGCH   C--- 3'
3' ---C   HCGDG--- 5' 		AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII
Synechocystis sp. 6701 5' CCNNGG
3' GGNNCC 		5' ---C   CNNGG--- 3'
3' ---GGNNC   C--- 5'
Synechococcus elongatus 5' CGCG
3' GCGC 		5' ---   CGCG--- 3'
3' ---GCGC   --- 5' 		AccII, Bsh1236I, Bsp123I, Bsp50I, BstFNI, BstUI, BtkI, Csp68KVI
Salmonella enteritidis PT16 5' CGGCCG
3' GCCGGC 		5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5' 		AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, XmaIII
Salmonella enteritidis PT14b 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Staphylococcus epidermidis 5' ATGCAT
3' TACGTA 		5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5' 		BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SspD5II, Zsp2I
Streptomyces exfoliatus 5' ACCWGGT
3' TGGWCCA 		5' ---A   CCWGGT--- 3'
3' ---TGGWCC   A--- 5'
Streptomyces exfoliatus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Streptomyces exfoliatus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Streptococcus faecalis zymogenes 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
Streptococcus faecalis ND547 5' GCATC
3' CGTAG 		5' ---GCATCN4N   NNNN--- 3'
3' ---CGTAGN4NNNNN   --- 5'
Streptococcus faecium 5' CTRYAG
3' GAYRTC 		5' ---C   TRYAG--- 3'
3' ---GAYRT   C--- 5'
Streptococcus faecalis 5' CTRYAG
3' GAYRTC 		5' ---C   TRYAG--- 3'
3' ---GAYRT   C--- 5'
2F03 Streptomyces fimbriatus 5' GGCCN5GGCC
3' CCGGN5CCGG 		5' ---GGCCNNNN   NGGCC--- 3'
3' ---CCGGN   NNNNCCGG--- 5'
Streptoverticillium flavopersicum 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		ApiI, BspMAI, BsuBI, CfrA4I, Psp23I, PstI, Sag23I, Srl5DI, YenI
5' GGCGCC
3' CCGCGG 		5' ---GGC   GCC--- 3'
3' ---CCG   CGG--- 5'
Streptomyces fradiae 274 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI
Streptomyces fradiae 303 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Streptomyces fulvissimus 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, BimI, Bsp119I, CbiI, Csp68KII, PlaII, Ssp1I, SviI
Streptomyces griseoruber 5' GCGATCGC
3' CGCTAGCG 		5' ---GCGAT   CGC--- 3'
3' ---CGC   TAGCG--- 5'
AsiSI,  RgaI, SfaAI 
3DPG 3N7B 3N78 3MGY 3MG6 Streptomyces griseus 5' CRCCGGYG
3' GYGGCCRC 		5' ---CR   CCGGYG--- 3'
3' ---GYGGCC   RC--- 5'
Streptomyces griseus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Staphylococcus intermedius 6H 5' GGGTC
3' CCCAG 		5' ---GG   GTC--- 3'
3' ---CCCAG   --- 5'
5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI
Streptomyces lavendulae 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI
5' CCWGG
3' GGWCC 		5' ---   CCWGG--- 3'
3' ---GGWCC   --- 5' 		AeuI, AglI, ApaORI, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI
Streptomyces lusitanus 1777 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
Serratia marcescens Sb 5' CCCGGG
3' GGGCCC 		5' ---CCC   GGG--- 3'
3' ---GGG   CCC--- 5' 		AhyI, CfrJ4I, EaeAI, EclRI, Pac25I, PspAI, TspMI, XcyI, XmaI, XmaCI
Streptococcus milleri S 5' ATTTAAAT
3' TAAATTTA 		5' ---ATTT   AAAT--- 3'
3' ---TAAA   TTTA--- 5'
5' CAYN4RTG
3' GTRN4YAC 		5' ---CAYNN   NNRTG--- 3'
3' ---GTRNN   NNYAC--- 5'
Stenotrophomonas maltophilia 5' CTYRAG
3' GARYTC 		5' ---C   TYRAG--- 3'
3' ---GARYT   C--- 5'
5' CCCGC
3' GGGCG 		5' ---CCCGCNNNN   NN--- 3'
3' ---GGGCGNNNNNN   --- 5'
Streptococcus mutans E 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI
Sphaerotilus natans 5' TACGTA
3' ATGCAT 		5' ---TAC   GTA--- 3'
3' ---ATG   CAT--- 5'
Streptomyces niveus 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, MvaI, SspAI
5' GTGCAC
3' CACGTG 		5' ---G   TGCAC--- 3'
3' ---CACGT   G--- 5' 		Alw44I, ApaLI, VneI
Streptoverticillium olivoverticillatum 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AccEBI, AliI, ApaCI, BamHI, Bce751I, CelI, OkrAI, SolI, SurI
Streptomyces olivochromogenes ST10179 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI
5' GCATGC
3' CGTACG 		5' ---GCATG   C--- 3'
3' ---C   GTACG--- 5'
Sphaerotilus natans 5' ACTAGT
3' TGATCA 		5' ---A   CTAGT--- 3'
3' ---TGATC   A--- 5' 		AhII, AclNI, BcuI
[7] Streptomyces phaeochromogenes 5' GCATGC
3' CGTACG 		5' ---GCATG   C--- 3'
3' ---C   GTACG--- 5'
5' CGTACG
3' GCATGC 		5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'
5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BanIII, BbvAII, BdiI, BscI, Bsa29I, BspJI, ClaI, LcaI, PgaI, Ssp27144I
5' TCGCGA
3' AGCGCT 		5' ---TCG   CGA--- 3'
3' ---AGC   GCT--- 5'
Streptomyces pulveraceus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Streptomyces sp. 5' GCCCGGGC
3' CGGGCCCG 		5' ---GCCC   GGGC--- 3'
3' ---CGGG   CCCG--- 5'
5' GCCGGC
3' CGGCCG 		5' ---G   CCGGC--- 3'
3' ---CGGCC   G--- 5'
Selenomonas ruminantium 5 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		AliAJI, Asp713I, BspMAI, BsuBI, CfrA4I, Pfl21I, PstI, SflI, Srl5DI
Selenomonas ruminantium 32 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
Selenomonas ruminantium 56 5' CTRYAG
3' GAYRTC 		5' ---C   TRYAG--- 3'
3' ---GAYRT   C--- 5'
Selenomonas ruminantium 18D 5' TTTAAA
3' AAATTT 		5' ---TTT   AAA--- 3'
3' ---AAA   TTT--- 5' 		AhaIII, DraI, PauAII
Selenomonas ruminantium 5' ATTAAT
3' TAATTA 		5' ---AT   TAAT--- 3'
3' ---TAAT   TA--- 5' 		AseI, AsnI, BpoAI, PshBI, VspI
Selenomonas ruminantium 30 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, SseBI, SteI, StuI
Streptomyces scabies 5' AAGCTT
3' TTCGAA 		5' ---A   AGCTT--- 3'
3' ---TTCGA   A--- 5'
Staphylococcus sp. L1 5' GANTC
3' CTNAG 		5' ---G   ANTC--- 3'
3' ---CTNA   G--- 5'
Sporosarcina sp. 9 5' AATT
3' TTAA 		5' ---   AATT--- 3'
3' ---TTAA   --- 5'
Streptomyces sp. RH232 5' CGCCGGCG
3' GCGGCCGC 		5' ---CG   CCGGCG--- 3'
3' ---GCGGCC   GC--- 5'
Streptomyces sp. 5' GGGWCCC
3' CCCWGGG 		5' ---GG   GWCCC--- 3'
3' ---CCCWG   GG--- 5'
Streptomyces sp. 8387 5' CCTGCAGG
3' GGACGTCC 		5' ---CCTGCA   GG--- 3'
3' ---GG   ACGTCC--- 5'
Streptomyces sp. 5' AGGWCCT
3' TCCWGGA 		5' ---AG   GWCCT--- 3'
3' ---TCCWG   GA--- 5'
Streptomyces sp. 5' GGCGCC
3' CCGCGG 		5' ---GG   CGCC--- 3'
3' ---CCGC   GG--- 5'
Streptomyces sp. 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SteI, StuI
5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI
Staphylococcus sciuri RFL1 5' CCGC
3' GGCG 		5' ---C   CGC--- 3'
3' ---GGC   G--- 5' 		AciI
Staphylococcus sp. A 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BscFI, BspAI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI
Staphylococcus sp. B 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI
5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII, MvaI
Shigella sonnei 47 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
Shigella sonnei 47 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
Sphaerotilus sp. 5' AATATT
3' TTATAA 		5' ---AAT   ATT--- 3'
3' ---TTA   TAA--- 5'
Streptomyces sp. RFL1 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, BimI, Bsp119I, CbiI, MlaI, PlaII, SfuI, SspRFI, SviI
Streptomyces sp. BMTU 4800 5' TGTACA
3' ACATGT 		5' ---T   GTACA--- 3'
3' ---ACATG   T--- 5'
AauI,  BsmRI,  Bsp1407I,
BsrGI, BstAUI, SspBI
Streptomyces sp. 5230 5' GACGTC
3' CTGCAG 		5' ---GACGT   C--- 3'
3' ---C   TGCAG--- 5' 		AatII, ZraI
Synechococcus sp. 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BanIII, BavCI, BbvAII, BscI, BspJI, BspOVII, BstNZ169I, ClaI, LcaI
Synechococcus sp. 5' CCWGG
3' GGWCC 		5' ---   CCWGG--- 3'
3' ---GGWCC   --- 5' 		AeuI, AorI, Bse17I, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI
Streptomyces sp. 5' TGTACA
3' ACATGT 		5' ---T   GTACA--- 3'
3' ---ACATG   T--- 5'
AauI,  BsmRI,  Bsp1407I,
BsrGI, BstAUI, Ssp4800I
Streptomyces sp. 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
Staphylococcus sp. D5 5' GGTGA
3' CCACT 		5' ---GGTGAN7N   --- 3'
3' ---CCACTN7N   --- 5' 		AsuHPI, HphI
Staphylococcus sp. D5 5' ATGCAT
3' TACGTA 		5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5' 		Csp68KIII, EcoT22I, Mph1103I, NsiI, PinBI, Ppu10I, SepI, Zsp2I
Synechococcus sp. RF-1 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, BimI, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I, SviI
Staphylococcus saprophyticus B6 5' GTTAAC
3' CAATTG 		5' ---GTT   AAC--- 3'
3' ---CAA   TTG--- 5'
5' GAGCTC
3' CTCGAG 		5' ---GAGCT   C--- 3'
3' ---C   TCGAG--- 5'
5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Streptomyces sp. ST-12 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		Asp713I, BloHII, BspMAI, BsuBI, CfuII, Psp23I, PstI, SflI, Srl5DI
Streptomyces tendae 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, StuI
5' GGTACC
3' CCATGG 		5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5' 		Acc65I, AhaB8I, Asp718I, KpnI,
Streptococcus thermophilus ST117 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, SspAI
Streptococcus thermophilus 5' CCCG
3' GGGC 		5' ---CCCGNNNN   NNNN--- 3'
3' ---GGGCNNNNNNNN   --- 5'
Streptococcus thermophilus 134 5' CCGG
3' GGCC 		5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
Streptococcus thermophilus CNRZ368 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI
Streptomyces thermodiastaticus 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI
Streptococcus sanguis 54 5' GGATG
3' CCTAC 		5' ---GGATGN8NN   NNNN--- 3'
3' ---CCTACN8NNNNNN   --- 5'
[8] Streptomyces tubercidicus 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI
Salmonella typhi 5' CCWWGG
3' GGWWCC 		5' ---C   CWWGG--- 3'
3' ---GGWWC   C--- 5'
Salmonella typhi D4 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
Sulfolobus acidocaldarius 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
Sulfolobus islandicus REN2HI 5' GCWGC
3' CGWCG 		5' ---G   CWGC--- 3'
3' --- CGWC   G--- 5' 		AceI, ApeKI, Taq52I, TseI
5' CGTACG
3' GCATGC 		5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'
Sporosarcina ureae 2 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AccEBI, AliI, ApaCI, AsiI, BamHI, Bce751I, OkrAI, RspLKII, SolI
5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, Bpu14I, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I
Staphylococcus warneri 5' ATTTAAAT
3' TAAATTTA 		5' ---ATTT   AAAT--- 3'
3' ---TAAA   TTTA--- 5'

Notes[]

  1. ^ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
  2. ^ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
  3. ^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
  4. ^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
  5. ^ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
  6. ^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
  7. ^ a b c d Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3.
  8. ^ Shimotsu H, Takahashi H, Saito H (November 1980). "A new site-specific endonuclease StuI from Streptomyces tubercidicus". Gene. 11 (3–4): 219–25. doi:10.1016/0378-1119(80)90062-1. PMID 6260571.
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