Methylenetetrahydrofolate dehydrogenase (NAD+)

In enzymology, a methylenetetrahydrofolate dehydrogenase (NAD⁺) (EC 1.5.1.15) is an enzyme that catalyzes a chemical reaction.

5,10-methylenetetrahydrofolate + NAD⁺ ${\displaystyle \rightleftharpoons }$ 5,10-methenyltetrahydrofolate + NADH + H⁺

Thus, the two substrates of this enzyme are 5,10-methylenetetrahydrofolate and NAD⁺, whereas its 3 products are 5,10-methenyltetrahydrofolate, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 5,10-methylenetetrahydrofolate:NAD⁺ oxidoreductase. This enzyme is also called methylenetetrahydrofolate dehydrogenase (NAD⁺). This enzyme participates in one carbon pool by folate.

Structural studies[]

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1EDZ and 1EE9.

References[]

• Moore MR, O'Brien WE, Ljungdahl LG (1974). "Purification and characterization of nicotinamide adenine dinucleotide-dependent methylenetetrahydrofolate dehydrogenase from Clostridium formicoaceticum". J. Biol. Chem. 249 (16): 5250–3. PMID 4153026.