Methylenetetrahydromethanopterin dehydrogenase

In enzymology, a methylenetetrahydromethanopterin dehydrogenase (EC 1.5.98.1) is an enzyme that catalyzes the chemical reaction

5,10-methylenetetrahydromethanopterin + coenzyme F₄₂₀ ${\displaystyle \rightleftharpoons }$ 5,10-methenyltetrahydromethanopterin + reduced coenzyme F₄₂₀

Thus, the two substrates of this enzyme are and coenzyme F420, whereas its two products are and reduced coenzyme F420.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with other acceptors. The systematic name of this enzyme class is 5,10-methylenetetrahydromethanopterin:coenzyme-F420 oxidoreductase. Other names in common use include N5,N10-methylenetetrahydromethanopterin dehydrogenase, and 5,10-methylenetetrahydromethanopterin dehydrogenase. This enzyme participates in folate biosynthesis.

Structural studies[]

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1QV9, 1U6I, 1U6J, and 1U6K.

References[]

• Hartzell PL, Zvilius G, Escalante-Semerena JC, Donnelly MI (1985). "Coenzyme F420 dependence of the methylenetetrahydromethanopterin dehydrogenase of Methanobacterium thermoautotrophicum". Biochem. Biophys. Res. Commun. 133 (3): 884–90. doi:10.1016/0006-291X(85)91218-5. PMID 4084309.
• GD; Geerts, WJ; Keltjens, JT; Van Der Drift, C; Vogels, GD (1991). "Purification and properties of 5,10-methylenetetrahydromethanopterin dehydrogenase and 5,10-methylenetetrahydromethanopterin reductase, two coenzyme F420-dependent enzymes, from Methanosarcina barkeri". Biochim. Biophys. Acta. 1079 (3): 293–302. doi:10.1016/0167-4838(91)90072-8. PMID 1911853.