tRNA guanosine-2'-O-methyltransferase

In enzymology, a tRNA guanosine-2'-O-methyltransferase (EC 2.1.1.34) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanosine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing 2'-O-methylguanosine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA guanosine-2'-O-methyltransferase. Other names in common use include transfer ribonucleate guanosine 2'-methyltransferase, tRNA guanosine 2'-methyltransferase, tRNA (guanosine 2')-methyltransferase, tRNA (Gm18) 2'-O-methyltransferase, tRNA (Gm18) methyltransferase, tRNA (guanosine-2'-O-)-methyltransferase, and S-adenosyl-L-methionine:tRNA (guanosine-2'-O-)-methyltransferase.

Structural studies[]

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1V2X and 1ZJR.

References[]

• Gefter ML (1969). "The in vitro synthesis of 2'-omethylguanosine and 2-methylthio 6N (gamma,gamma, dimethylallyl) adenosine in transfer RNA of Escherichia coli". Biochem. Biophys. Res. Commun. 36 (3): 435–41. doi:10.1016/0006-291X(69)90583-X. PMID 4898378.
• Kumagai I, Watanabe K, Oshima T (1980). "Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27". Proc. Natl. Acad. Sci. U.S.A. 77 (4): 1922–6. Bibcode:1980PNAS...77.1922K. doi:10.1073/pnas.77.4.1922. PMC 348621. PMID 6990416.
• Kumagai I, Watanabe K (1998). "Substrate recognition of tRNA (Guanosine-2'-)-methyltransferase from Thermus thermophilus HB27". J. Biol. Chem. 273 (40): 25721–7. doi:10.1074/jbc.273.40.25721. PMID 9748240.