1,4-lactonase

From Wikipedia, the free encyclopedia
1,4-lactonase
Identifiers
EC no.3.1.1.25
CAS no.37278-38-9
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a 1,4-lactonase (EC 3.1.1.25) is an enzyme that catalyzes the generic chemical reaction:

a 1,4-lactone + H2O a 4-hydroxyacid

Thus, the two substrates of this enzyme are 1,4-lactone and H2O, whereas its product is .

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is 1,4-lactone hydroxyacylhydrolase. This enzyme is also called gamma-lactonase. This enzyme participates in galactose metabolism and . It employs one cofactor, calcium.

Structural studies[]

As of late 2007, three structures have been solved for this class of enzymes, with PDB accession codes 2DG0, 2DG1, and 2DSO.

Applications[]

In a study by Chen et al. a 1,4-lactonase was expressed in E. coli and used as a highly efficient biocatalyst for asymmetric synthesis of chiral compounds.[1]

References[]

  1. ^ Chen, Bing; Fan, Li-Qiang; Xu, Jian-He; Zhao, Jian; Zhang, Xian; Ouyang, Li-Ming (October 2010). "Biocatalytic properties of a recombinant Fusarium proliferatum lactonase with significantly enhanced production by optimal expression in Escherichia coli". Applied Biochemistry and Biotechnology. 162 (3): 744–756. doi:10.1007/s12010-009-8819-1. ISSN 1559-0291. PMID 19876606.
  • Fishbein WN, Bessman SP (1966). "Purification and properties of an enzyme in human blood and rat liver microsomes catalyzing the formation and hydrolysis of gamma-lactones. I. Tissue localization, stoichiometry, specificity, distinction from esterase". J. Biol. Chem. 241 (21): 4835–41. PMID 4958984.
  • Fishbein WN, Bessman SP (1966). "Purification and properties of an enzyme in human blood and rat liver microsomes catalyzing the formation and hydrolysis of gamma-lactones. II. Metal ion effects, kinetics, and equilibra". J. Biol. Chem. 241 (21): 4842–7. PMID 4958985.


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