Sin Nombre orthohantavirus

Sin Nombre orthohantavirus
Transmission electron micrograph of Sin Nombre orthohantavirus
Virus classification
(unranked):	Virus
Realm:	Riboviria
Kingdom:	Orthornavirae
Phylum:	Negarnaviricota
Class:	Ellioviricetes
Order:	Bunyavirales
Family:	Hantaviridae
Genus:	Orthohantavirus
Species:	Sin Nombre orthohantavirus
Member viruses
Blue River virus Monongahela virus New York orthohantavirus
Synonyms
Four Corners virus Sin Nombre hantavirus Sin Nombre virus

Sin Nombre orthohantavirus (SNV) (from Spanish, meaning "without a name") is the prototypical etiologic agent of hantavirus cardiopulmonary syndrome (HCPS).^[1]

Discovered in 1993 near the Cañon de la Muerte on the Navajo Reservation, it was originally named the Muerto Canyon hantavirus, in keeping with the convention for naming new pathogens.^[2] However, the Navajo Nation objected to the name in 1994.^[3] It was also near the Four Corners point in the United States, so the virologists then tried naming it the "Four Corners virus". The name was changed after local residents raised objections.^[4] In frustration, the virologists changed it to Sin Nombre, meaning "without a name" in Spanish.

History[]

It was first isolated in 1993 from rodents collected near the home of one of the initial patients with hantavirus pulmonary syndrome (HPS) in the Four Corners region of the western United States. Isolation was achieved through blind passage in Peromyscus maniculatus (deer mouse) and subsequent adaptation to growth in Vero E6 cells. Additional viral strains have also been isolated from P. maniculatus associated with a fatal case in California and P. leucopus from the vicinity of probable infection of a New York case. Black Creek Canal virus was isolated from S. hispidus collected near the residence of a human case in Dade County, Florida. Another etiologic agent of HCPS, Bayou virus, was first isolated from the vicinity of Monroe, Louisiana.^[5]

Epidemiology[]

SNV occurs wherever its reservoir rodent carrier, the deer mouse Peromyscus maniculatus,^[6] is found, which includes essentially the entire populated area of North America, except for the far southeastern region from eastern Texas through Florida, Alaska, and the far northern reaches of Canada. SNV and HCPS are especially common in western states; peak incidences for HCPS have been reported in regions in which there is a lot of contact between humans and mice (New Mexico, Arizona) and in states with exceptionally large rural populations such as California. All of the western provinces of Canada have also reported cases. SNV can be contracted through the inhalation of virus-contaminated deer mouse excreta.^{[citation needed]}

The case fatality ratio of SNV-induced HCPS in the USA was reported to be about 66.7% (CDC, 1993). However, since that time the case fatality ratio has steadily declined as more mild cases came to be recognized. By 2007 the CFR had declined to about 35%.^{[citation needed]}

Virus sequencing[]

The entire genomic sequence of SNV has subsequently been determined by using RNA extracted from autopsy material as well as RNA extracted from cell culture-adapted virus. The L RNA is 6562 nucleotides (nt) in length; the M RNA is 3696 nt long; and the S RNA is 2059 to 2060 nt long. When the prototype sequence (NMH15) of SNV detected in tissues from an HPS case was compared with the sequence of the SNV isolate (NMR11; isolated in Vero E6 cells from Peromyscus maniculatus trapped in the residence of the same case), only 16 nucleotide changes were found, and none of these changes resulted in alterations in amino acid sequences of viral proteins. It had been assumed that in the process of adaptation to cell culture, selection of SNV variants which grow optimally in cell culture would occur, and selected variants would differ genetically from the parental virus. Though NMH10 and NMR11 are identical in protein sequence, nucleotide substitutions in nontranslated regions of the genome could be responsible for altered viral phenotypes, as could changes in protein glycosylation or virus membrane components.^{[citation needed]}

The nested RT-PCR assay developed during the initial HCPS outbreak provided a rapid method for the genetic characterization of novel hantaviruses that did not require a virus isolate. Numerous new hantaviruses have been detected by RT-PCR in rodent tissues but have yet to be associated with human disease. These include El Moro Canyon virus associated with the western harvest mouse, Reithrodontomys megalotis, Tula virus with Microtus arvalis and M. rossiaemeridionalis, Rio Segundo virus with the Mexican harvest mouse, R. mexicanus, Isla Vista virus with the California vole, M. californicus, and Prospect Hill-like viruses in Microtus species.^{[citation needed]}

See also[]

• 1993 Four Corners hantavirus outbreak
• Hantavirus

References[]

External links[]

• Virology - CDC Hantaviruses
• Clinical and Lab Recognition and other info